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  • F4055 - Anti-FMR1 (C-terminal) antibody produced in rabbit

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F4055 Sigma-Aldrich

Anti-FMR1 (C-terminal) antibody produced in rabbit

~1.0 mg/mL, affinity isolated antibody, buffered aqueous solution

Synonym: Anti-FMRP, Anti-Fragile X Mental Retardation Protein

  •  NACRES NA.41

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Properties

Related Categories Alphabetical Index, Antibodies, Antibodies for Epigenetics and Nuclear Signaling, Antibodies for Gene Regulation, Antibodies for RNA Regulation and Translation,
conjugate   unconjugated
clone   polyclonal
biological source   rabbit
application(s)   immunoprecipitation (IP): 5-10 μg using HEK-293T cells lysate
  indirect immunofluorescence: 2-5 μg/mL using methanol-acetone fixed heat-shocked NIH3T3 cells
  western blot: 1-2 μg/mL using HEK-293T cell lysate
  western blot: 2-4 μg/mL using RAT1 cell lysate
species reactivity   rat, mouse, human
mol wt   antigen ~80 kDa
form   buffered aqueous solution
shipped in   dry ice
storage temp.   −20°C
antibody form   affinity isolated antibody
antibody product type   primary antibodies
Quality Level   200
concentration   ~1.0 mg/mL
UniProt accession no.   Q06787
Gene Information   human ... FMR1(2332)
mouse ... Fmr1(14265)
rat ... Fmr1(24948)

Description

General description

FMR1 is a RNA binding protein expressed mainly in brain, neurons, placenta, testes and lymphocytes. Defect in FMR1 can lead to Fragile X Mental Retardation Syndrome due to lack of expression of FMR1 or expression of a mutant protein that cannot bind RNA. Anti-FMR1 (C-terminal) antibody can be used in immunofluorescence staining. Rabbit anti-FMR1 (C-terminal) antibody reacts specifically with FMR1.

The FMR1 protein (fragile X mental retardation 1 protein) can bind to RNA. It contains two heterogeneous nuclear ribonucleoprotein K homology (KH) domains and one RGG box. Two proteins named FXR1 and FXR2 interact with FMR1. The protein is highly expressed in brain and testis.

Immunogen

synthetic peptide corresponding to amino acids 606-623 of human FMR1, conjugated to KLH. The corresponding sequence is highly conserved (1 amino acid substitution) in rat and mouse.

Application

Anti-FMR1 (C-terminal) antibody produced in rabbit has been used in: western blotting, immunoprecipitation, immunofluorescence, immunoblotting .

Physical form

Solution in 0.01 M phos­phate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
Protocols & Articles

Articles

Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy

Protocols

Western Blot Protocol | Immunoblotting Protocol

Western Blotting refers to the electrophoretic transfer of proteins from sodium dodecyl sulfate polyacrylamide gels to sheets of PVDF or nitrocellullose membrane, followed by immunodetection of prote...
Keywords: AGE, Buffers, Cell disruption, Detection methods, Detergents, Dialysis, Electroblotting, Electrophoresis, Enzyme activity, Gel electrophoresis, Immunoprecipitation, PAGE, Protein extraction, Purification, Sample preparations, Western blot

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Peer-Reviewed Papers
15

References

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