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F4129 Sigma-Aldrich

Fibrinogen from human plasma

55-75% protein, ≥60% of protein is clottable

Synonym: Factor I



Related Categories Application Index, Biochemicals and Reagents, Coagulation Proteins and Reagents, Enzymes, Inhibitors, and Substrates, Fibrinogen,
quality   ≥60% of protein is clottable
mol wt   α-chain 63.5 kDa
  β-chain 56 kDa
  γ chain 47 kDa (about 4% carbohydrate content)
  soluble dimer 340 kDa
concentration   55-75% protein
solubility   0.9% NaCl: soluble 10 mg/mL
storage temp.   −20°C
Gene Information   human ... FGA(2243), FGB(2244), FGG(2266)


General description

Fibrinogen, or Factor I, is a blood protein that is involved in clotting and is converted to fibrin by thrombin. Fibrinogen has an approximate molecular weight of 340 kDa.


Fibrinogen was used in velocity variation assessment of red blood cell aggregation using spectral domain Doppler optical coherence tomography. It was also used to study the nano-bio interaction between graphite oxide nanoparticles and human blood components.

Biochem/physiol Actions

Fibrinogen is an acute phase protein that is part of the coagulation cascade of proteins. The end result of the cascade is the production of thrombin that converts fibrinogen to fibrin. Thrombin rapidly proteolyses fibrinogen, releasing fibrinopeptide A. The loss of this small peptide is not sufficient to make the resulting fibrin molecule insoluble, but it tends to form complexes with adjacent fibrin and fibrinogen molecules. Thrombin then cleaves a second peptide, fibrinopeptide B, from fibrin and the fibrin monomers formed then polymerize spontaneously to form an insoluble gel. The polymerized fibrin is held together by noncovalent and electrostatic forces and stabilized by the transamidating enzyme, factor XIIIa, that is produced by the action of thrombin on factor XIII. The insoluble fibrin aggregates (clots) and aggregated platelets then block the damaged blood vessel and prevent further bleeding. The amount of fibrinogen in the plasma can serve as a nonspecific indicator of whether or not an inflammatory process is present in the body. Fibrinogen from any mammalian source will be cleaved by thrombin from any mammalian source.


Source material has tested negative for HIV and HBsAg.

Physical form

Lyophilized powder containing ~15% sodium citrate and ~20% sodium chloride.

Analysis Note

Protein determined by biuret

Safety & Documentation

Safety Information

NONH for all modes of transport
WGK Germany 


Certificate of Analysis (COA)

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Certificate of Origin (COO)

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Protocols & Articles


Plasma Derived Proteins and Enzymes

Despite their complexity, blood and plasma are abundant biological resources for the discovery of drug targets and biomarkers for human disease. It is estimated that plasma may contain as many as 40,...
BioFiles 2006, 1.5, 2.
Keywords: Applications, Gas chromatography, Type

Peer-Reviewed Papers


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