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  • F4307 - Anti-FOXI1 (156-170) antibody produced in rabbit

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F4307 Sigma-Aldrich

Anti-FOXI1 (156-170) antibody produced in rabbit

IgG fraction of antiserum, buffered aqueous solution

Synonym: Anti-FKH10, Anti-FKHL10, Anti-FREAC6, Anti-Forkhead box protein I1 isoform 2, Anti-HFH3

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Properties

Related Categories Alphabetical Index, Antibodies, Antibodies for Epigenetics and Nuclear Signaling, Antibodies to Transcription Factors, EA - G999,
conjugate   unconjugated
clone   polyclonal
biological source   rabbit
application(s)   western blot: 1:500-1:2,000
species reactivity   human
mol wt   antigen ~31 kDa
form   buffered aqueous solution
shipped in   dry ice
storage temp.   −20°C
antibody form   IgG fraction of antiserum
antibody product type   primary antibodies
UniProt accession no.   Q12951
Gene Information   human ... FOXI1(2299)

Description

Immunogen

synthetic peptide corresponding to amino acids 156-170 of human FOXI1

Application

Anti-FOXI1 (156-170) antibody is suitable for western blot analysis at a dilution of 1:500-1:2,000.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Biochem/physiol Actions

FOXI1 belongs to the superfamily of forkhead transcription factors (FOX). It is characterized by a common forkhead domain, a variant of the helix-turn-helix motif. It has homology to the winged helix DNA binding domain. It has been demonstrated that HFH-3 expression is restricted to the epithelium of the renal distal convoluted tubules. FOXI1 is associated in cell fate determination during embryonic pattern formation, in organogenesis, and in cell-type-specific gene expression.

Safety & Documentation

Safety Information

Safety Information for this product is unavailable at this time.

Documents

Certificate of Analysis (COA)

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Protocols & Articles

Articles

Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy

Protocols

Western Blot Protocol | Immunoblotting Protocol

Western Blotting refers to the electrophoretic transfer of proteins from sodium dodecyl sulfate polyacrylamide gels to sheets of PVDF or nitrocellullose membrane, followed by immunodetection of prote...
Keywords: AGE, Buffers, Cell disruption, Detection methods, Detergents, Dialysis, Electroblotting, Electrophoresis, Enzyme activity, Gel electrophoresis, Immunoprecipitation, PAGE, Protein extraction, Purification, Sample preparations, Western blot

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Peer-Reviewed Papers
15

References

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