• USA Home
  • F4641 - Monoclonal Anti-Human IgG3−FITC antibody produced in mouse

EMAIL THIS PAGE TO A FRIEND
F4641 Sigma-Aldrich

Monoclonal Anti-Human IgG3−FITC antibody produced in mouse

clone HP-6050, purified from hybridoma cell culture

Synonym: Monoclonal Anti-Human IgG3

  •  NACRES NA.46

Purchase

Properties

Related Categories Alphabetical Index, Antibodies, FITC Antibodies, HU-HZ, Human IgG Secondary Antibodies and Conjugates,
conjugate   FITC conjugate
clone   HP-6050, monoclonal
biological source   mouse
application(s)   dot immunobinding: 1:64
  particle immunofluorescence: 1:8
form   buffered aqueous solution
shipped in   dry ice
storage temp.   −20°C
antibody form   purified immunoglobulin
isotype   IgG1
Quality Level   200
antibody product type   secondary antibodies
storage condition   protect from light

Description

General description

Human Immunoglobulin G consists of four subclasses, namely IgG1, IgG2, IgG3, and IgG4, named γ1, γ2, γ3, and γ4, respectively. The primary structure of the antibody contains disulfide bonds involved in linking the two heavy chains,and linking the heavy and light chains. IgG3, a pro-inflammatory antibody, appears first in the course of a viral infection and induces effector functions.

Specificity

The antibody is specific for human IgG3 and is non-reactive with other IgG subclasses. This clone has been recommended by the IUIS/WHO study for its specificity and reactivity in a wide range of assay protocols and techniques.

Application

Monoclonal Anti-Human IgG3-FITC antibody produced in mouse has been used in immunohistochemistry and indirect immunofluorescence.

Physical form

Solution in 0.01 M phosphate buffer, pH 8.0, containing 1% inactivated bovine serum albumin and 15 mM sodium azide.

Storage and Stability

For continuous use, store at 2-8 °C for up to one month.
For extended storage, solution may be frozen in working aliquots. Repeated freezing and thawing is not recommended. If slight turbidity occurs upon prolonged storage, clarify by centrifugation before use.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Other Notes

In order to obtain best results, it is recommended that each individual user determine working dilutions by titration assay.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
nwg
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable
Protocols & Articles

Articles

Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy

How to Choose a Secondary Antibody

The following information is provided to help you decide which secondary antibody may be best for your particular application.
Keywords: Amplification, Enzyme-linked immunosorbent assay, Flow cytometry, Immobilization, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Western blot

Secondary Antibodies, Conjugates and Kits

Secondary antibodies are polyclonal or monoclonal antibodies that bind to primary antibodies or antibody fragments, such as the Fc or Fab regions. They are typically labeled with probes that make the...
Keywords: Absorption, Adsorption, Amplification, Bacterial conjugations, Cancer, Digestions, Electrophoresis, Enzyme-linked immunosorbent assay, Flow cytometry, Gel electrophoresis, Gene expression, Hormones, Immobilization, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Immunology, Immunoprecipitation, Infrared spectroscopy, Microbiology, Microscopy, Purification, Vitamins, Western blot

Related Content

Antibody Explorer | Buy Primary Antibodies & Secondary Antibodies

Primary, Secondary and Recombinant Monoclonal Antibodies Providing highly cited primary and secondary antibodies, we have you covered for your ELISA, western blot, immunohistochemistry or other assay...
Keywords: Alzheimer Disease, Cancer, Chromatin immunoprecipitation, Diagnostic, Enzyme-linked immunosorbent assay, Flow cytometry, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Neuroscience, Western blot

Peer-Reviewed Papers
15

References

Related Products

Technical Service:

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Bulk Ordering & Pricing:

Need larger quantities for your development, manufacturing or research applications?