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F4759 Sigma-Aldrich

Fibronectin bovine plasma

powder, BioReagent, suitable for cell culture

Synonym: Fibronectin

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Properties

Related Categories Adhesion, Attachment, and Matrix Factors for Stem Cell Expansion, Application Index, Attachment Factors, Attachments Factors, Biochemicals and Reagents,
Quality Level   200
biological source   bovine plasma
sterility   sterile
product line   BioReagent
form   powder
mol wt   ~450 kDa
packaging   pkg of 1 mg
  pkg of 2 mg
  pkg of 5 mg
application(s)   cell culture | mammalian: suitable
surface coverage   1‑5 μg/cm2
solubility   H2O: 1 mg/mL at 37 °C (Store reconstituted solution in working aliquots at -20°C or lower.)
shipped in   dry ice
storage temp.   −20°C
Gene Information   bovine ... FN1(2807940)

Description

General description

Fibronectin (FN) is a multifunctional glycoprotein, the gene of which is localized to human chromosome 2q34-36. This gene spans 75kb and contains 50 exons. It exists as two major isoforms, one soluble and one insoluble form. The former is present in plasma, whereas the insoluble form resides in tissues and extracellular matrix (ECM) of cartilage. There are around 20 different forms of FN due to alternative splicing of a variable region and two type III exons called Extra Domains A and B.

Fibronectin is a glycoprotein with multiple domains. It comprises a N-terminal, central binding domain and a heparin-binding domain. Bovine plasma fibronectin has polypeptide chains with disulfide linkage and corresponds to a molecular weight of 220 and 215 kDa.

Application

Fibronectin from bovine plasma is used for the following applications:
• Used for coating the glass coverslips for the culture of decidual stromal cells (DEC)
• Cell movement assays (culture plates were coated with fibronectin)
• Cell spreading and immunofluorescence
• Protein specificity
• Integration of sensor films into microfluidic chips
• Histological and Immunohistochemical (IHC) analyses
• Used in primary cell cultures from patients with lung carcinoma

Biochem/physiol Actions

Fibronectin is a regulator of protein scaffolding. It coordinates cellular processes like platelet functions, wound-healing, adhesion, migration and aggregation.

Caution

As supplied, this product should be stored at -20°C and will remain active for at least 2 years.

Safety & Documentation

Safety Information

Personal Protective Equipment 
RIDADR 
NONH for all modes of transport
WGK Germany 
WGK 3
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable

Documents

Certificate of Analysis (COA)

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Certificate of Origin (COO)

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Protocols & Articles

Articles

Cancer Stem Cells: New Targets for Cancer Therapy

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Extracellular Matrix Proteins and tools for cell culture optimization

Animal cells and tissue culture techniques are constantly improved to optimize in vitro cell culture conditions. Extracellular Matrix (ECM) proteins coating, chemical or physical modification of the ...
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Fibronectin (FN)

Fibronectin (FN) is a multifunctional, extracellular matrix glycoprotein composed of two nearly identical disulfide bound polypeptides of molecular weight 220 kDa. Cellular fibronectin is structurall...
George Sitterley
BioFiles 2008, 3.8, 8.
Keywords: Adhesion, Cell culture, transformation

What is the Extracellular Matrix?

The extracellular matrix (ECM) is secreted by cells and surrounds them in tissues. It has long been understood to be the structural support for cells since its characteristics set the characteristics...
Keywords: Adhesion, Cell culture, Cellular processes, Epimerizations, Growth factors, Immobilization, Sulfations

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Peer-Reviewed Papers
15

References

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