• USA Home
  • F4772 - Monoclonal Anti-Cytokeratin Peptide 18−FITC antibody produced in mouse

EMAIL THIS PAGE TO A FRIEND
F4772 Sigma-Aldrich

Monoclonal Anti-Cytokeratin Peptide 18−FITC antibody produced in mouse

clone CY-90, purified immunoglobulin, buffered aqueous solution

Synonym: Monoclonal Anti-Cytokeratin Peptide 18

Purchase

Properties

Related Categories Alphabetical Index, Antibodies, Antibodies for Cell Biology, Antibodies to Cell and Organelle Proteins, Antibodies to Cytokeratins,
conjugate   FITC conjugate
clone   CY-90, monoclonal
biological source   mouse
application(s)   direct immunofluorescence: 1:100 using formalin-fixed, paraffin-embedded sections of human or animal tissues
  immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
species reactivity   wide range
form   buffered aqueous solution
shipped in   dry ice
storage temp.   −20°C
antibody form   purified immunoglobulin
isotype   IgG1
Quality Level   200
antibody product type   primary antibodies

Description

General description

Broadly keratins are grouped into two types on the basis of their biochemical and immunological properties: Type II keratins are a family of basic proteins identified as K1 to K8, whereas type I keratins consists of K9 to K20, belong to the acidic group. It belongs to type I acrylic sub-family and is differentially expressed in various human tissues.

FITC Monoclonal Anti-Cytokeratin Peptide 18 is a purified mouse monoclonal antibody conjugated with fluorescein isothiocyanate (FITC) isomer I. Monoclonal Anti-Cytokeratin Peptide 18 (mouse IgG1 isotype) is derived from the CY-90 hybridoma produced by the fusion of mouse myeloma cells and splenocytes of immunized BALB/c mice. Cytokeratin 18 (CYK18) is a 45 kDa polypeptide, differentially expressed in various human tissues.

Immunogen

human epidermal carcinoma A-431 and MCF-7 human breast cancer cell lines.

Application

Monoclonal Anti-Cytokeratin Peptide 18 FITC antibody produced in mouse has been used in:
• direct immunofluorescent staining
• immunohistochemistry
• double immunofluorescence
• immunocytochemical and immuno?electron microscopic localization of keratins

Monoclonal Anti-Cytokeratin Peptide 18-FITC antibody:
• is suitable for immunohistochemistry to identify renal tubular epithelial cells in the extrarenal cells regeneration during acute renal failure
• is suitable for immunocytochemical and immuno-electron microscopic localisation of keratins in human materno-foetal interaction zone
• may be used for single or double labeling procedures

Physical form

Supplied as a solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% BSA with 15 mM sodium azide as a preservative.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Biochem/physiol Actions

Cytokeratins also called keratins (Ks) according to the new nomenclature system.These are known to be the major structural proteins of epithelial cells. It is heteropolymeric in nature i.e. in cells more than one keratin protein is always present. It belongs to type I acrylic sub-family and is differentially expressed in various human tissues. It maintains the structural integrity of extra-embryonic membrane epithelia. Broadly keratins are grouped into two types on the basis of their biochemical and immunological properties: Type II keratins are a family of basic proteins identified as K1 to K8, whereas type I keratins consists of K9 to K20, belong to the acidic group.

Monoclonal anti-cytokeratins are specific markers of epithelial cell differentiation and have been widely used as tools in tumor identification and classification. FITC Monoclonal Anti-Cytokeratin Peptide 18 is a chain specific antibody which can facilitate typing of normal, metaplastic and neoplastic cells. Cytokeratin 18 (CYK18) is a cytoskeletal protein associated with apoptosis. CK-18 is considered as a promising plasma biomarker to identify patients with HELLP (hemolysis, elevated liver enzymes, low platelets) syndrome.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable
Protocols & Articles

Articles

Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy

Related Content

Antibody Explorer | Buy Primary Antibodies & Secondary Antibodies

Primary, Secondary and Recombinant Monoclonal Antibodies Providing highly cited primary and secondary antibodies, we have you covered for your ELISA, western blot, immunohistochemistry or other assay...
Keywords: Alzheimer Disease, Cancer, Chromatin immunoprecipitation, Diagnostic, Enzyme-linked immunosorbent assay, Flow cytometry, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Neuroscience, Western blot

Peer-Reviewed Papers
15

References

Related Products

Technical Service:

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Bulk Ordering & Pricing:

Need larger quantities for your development, manufacturing or research applications?