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F5022 Sigma-Aldrich

Fibronectin-like Protein Polymer genetically engineered

lyophilized powder, autoclaved, BioReagent, suitable for cell culture

Synonym: ProNectin® F

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Properties

Related Categories Attachment Factors, Attachments Factors, Biochemicals and Reagents, Cell Culture, Core Bioreagents,
sterility   autoclaved
product line   BioReagent
form   lyophilized powder
mol wt   mol wt 75 kDa by gene sequence (differences are due to the unusual amino acid composition of the protein)
  mol wt ~110 kDa by SDS-PAGE
packaging   pkg of 1 mg
  pkg of 5 mg
solubility   4.5  M LiClO4: 1 mg/mL (stable for 12 mo. at RT.)
suitability   suitable for cell culture
storage temp.   room temp

Description

Application

Recommended as a cell culture substratum at 2-10 μg/cm2.

Other Notes

Positively-charged, protein polymer which incorporates multiple copies of the RGD cell attachment epitope from human fibronectin between repeated structural peptide units.

Packaging

Supplied with diluent in separate vial.

Reconstitution

Dilutions can be made to a working concentration with balanced salt solution; stable up to 2 days at RT. Coated labware is stable at least 4 mos at RT.

Legal Information

ProNectin is a registered trademark of Sanyo Chemical Industries, Ltd

Safety & Documentation

Safety Information

Symbol 
Signal word 
Danger
Hazard statements 
Precautionary statements 
RIDADR 
NONH for all modes of transport
WGK Germany 
3

Documents

Certificate of Analysis

Certificate of Origin


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Cell Culture Guide
Protocols & Articles

Articles

Extracellular Matrix Proteins and tools for cell culture optimization

Animal cells and tissue culture techniques are constantly improved to optimize in vitro cell culture conditions. Extracellular Matrix (ECM) proteins coating, chemical or physical modification of the ...
Keywords: Adhesion, Angiogenesis, Apoptosis, Asymmetric synthesis, Cancer, Cell attachment, Cell culture, Cell proliferation, Coagulation, Endocrinology, Growth factors, Hormones

Fibronectin (FN)

Fibronectin (FN) is a multifunctional, extracellular matrix glycoprotein composed of two nearly identical disulfide bound polypeptides of molecular weight 220 kDa. Cellular fibronectin is structurall...
George Sitterley
BioFiles 2008, 3.8, 8.
Keywords: Adhesion, Cell culture, transformation

What is the Extracellular Matrix?

The extracellular matrix (ECM) is secreted by cells and surrounds them in tissues. It has long been understood to be the structural support for cells since its characteristics set the characteristics...
Keywords: Adhesion, Cell culture, Cellular processes, Epimerizations, Growth factors, Immobilization, Sulfations

Related Content

Cell Culture Troubleshooting Guide

Cell culture has become one of the most fundamental techniques for modeling biological systems, and is of increasing importance in the biotechnology and pharmaceutical sectors as well as an essential...
Keywords: Antibiotics, Apoptosis, Cell attachment, Cell culture, Cryopreservation, Filtration, Growth factors, Pharmaceutical, Phase transitions

Peer-Reviewed Papers
15

References

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