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F5027 Sigma-Aldrich

Monoclonal Anti-IgD−FITC antibody produced in mouse

clone HJ9, purified immunoglobulin, buffered aqueous solution

Synonym: Monoclonal Anti-Human IgD

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Properties

Related Categories Alphabetical Index, Analytical Cytology, Anti-Immunoglobulins, Antibodies, Antibodies for Cell Biology,
conjugate   FITC conjugate
clone   HJ9, monoclonal
biological source   mouse
application(s)   flow cytometry: 10 μL using 1 × 106 cells
species reactivity   human
form   buffered aqueous solution
shipped in   wet ice
storage temp.   2-8°C
antibody form   purified immunoglobulin
isotype   IgG1
antibody product type   secondary antibodies
UniProt accession no.   P01880
Gene Information   human ... IGHD(3495)

Description

Specificity

Recognizes the delta chain of human IgD and is not reactive with heavy chains from human IgG1, IgG2, IgG3, IgG4, IgM, IgA, or kappa and lambda light chains.

Immunogen

human IgD

Application

Monoclonal Anti-IgD-FITC antibody is suitable for flow cytometry at a concentration of 10μL using 1×106 cells.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Biochem/physiol Actions

IgD is the major antigen receptor isotype coexpressed with IgM on the surface of human peripheral B cells. It is associated with early antibody response. It occupies approximately 0.25% of total immunoglobulins. In serum, it has shown that the catabolism of IgD is decreased at high concentrations. IgD molecule comprises of a long “hinge” region (between Fab and Fc) including three constant region domains. This makes the molecule very susceptible to proteolytic degradation with production of Fab and Fc fragments. It also helps to maintain the molecule flexibility that enhance antigen binding capacity. IgD is a potent inducer of tumor necrosis factor (TNF), Interlukin 1β(IL1β), and Interlukin IL1RN (IL1RN). It also accelerates the release of IL6, IL10 from peripheral blood mononuclear cells. Its increased level in serum causes hyper-IgD and periodic fever syndrome (HIDS).

Other Notes

IgD is expressed on peripheral B cells and is used as a pan-B cell marker.

Preparation Note

Prepared by conjugation to fluorescein isothiocyanate isomer I (FITC). This green dye is efficiently excited at 495 nm and emits at 525 nm.

Safety & Documentation

Safety Information

WGK Germany 
3

Documents

Certificate of Analysis (COA)

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Protocols & Articles

Articles

Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy

How to Choose a Secondary Antibody

The following information is provided to help you decide which secondary antibody may be best for your particular application.
Keywords: Amplification, Enzyme-linked immunosorbent assay, Flow cytometry, Immobilization, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Western blot

Secondary Antibodies, Conjugates and Kits

Secondary antibodies are polyclonal or monoclonal antibodies that bind to primary antibodies or antibody fragments, such as the Fc or Fab regions. They are typically labeled with probes that make the...
Keywords: Absorption, Adsorption, Amplification, Bacterial conjugations, Cancer, Digestions, Electrophoresis, Enzyme-linked immunosorbent assay, Flow cytometry, Gel electrophoresis, Gene expression, Hormones, Immobilization, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Immunology, Immunoprecipitation, Infrared spectroscopy, Microbiology, Microscopy, Purification, Vitamins, Western blot

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Peer-Reviewed Papers
15

References

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