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  • F5137 - Anti-Sheep IgG (whole molecule)−FITC antibody produced in rabbit

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F5137 Sigma-Aldrich

Anti-Sheep IgG (whole molecule)−FITC antibody produced in rabbit

IgG fraction of antiserum, buffered aqueous solution

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Properties

Related Categories Alphabetical Index, Antibodies, FITC Antibodies, Primary Antibodies, SG-SK,
conjugate   FITC conjugate
clone   polyclonal
biological source   rabbit
application(s)   immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:100
  indirect immunofluorescence: 1:100
form   buffered aqueous solution
storage temp.   −20°C
antibody form   IgG fraction of antiserum
antibody product type   secondary antibodies
storage condition   protect from light

Description

General description

IgG antibody subtype is the most abundant serum immunoglobulins of the immune system. It is secreted by B cells and is found in blood and extracellular fluids and provides protection from infections caused by bacteria, fungi and viruses. Maternal IgG is transferred to fetus through the placenta that is vital for immune defence of the neonate against infections. Anti-Sheep IgG (whole molecule)-FITC antibody is specific for sheep IgG. Rabbit Anti-Sheep IgG is conjugated to Fluorescein Isothiocyanate (FITC) in an alkaline reaction, then further purified to remove unbound FITC.

Immunogen

Purified sheep IgG

Application

Anti-Sheep IgG (whole molecule)-FITC antibody may be used for immunohistochemistry and immunofluorescence at a working antibody dilution of 1:100. The antibody was used for direct immunofluorescence of rat kidney sections at a dilution of 1:50.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
nwg

Documents

Certificate of Analysis (COA)

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Certificate of Origin (COO)

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Protocols & Articles

Articles

Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy

How to Choose a Secondary Antibody

The following information is provided to help you decide which secondary antibody may be best for your particular application.
Keywords: Amplification, Enzyme-linked immunosorbent assay, Flow cytometry, Immobilization, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Western blot

Secondary Antibodies, Conjugates and Kits

Secondary antibodies are polyclonal or monoclonal antibodies that bind to primary antibodies or antibody fragments, such as the Fc or Fab regions. They are typically labeled with probes that make the...
Keywords: Absorption, Adsorption, Amplification, Bacterial conjugations, Cancer, Digestions, Electrophoresis, Enzyme-linked immunosorbent assay, Flow cytometry, Gel electrophoresis, Gene expression, Hormones, Immobilization, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Immunology, Immunoprecipitation, Infrared spectroscopy, Microbiology, Microscopy, Purification, Vitamins, Western blot

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Peer-Reviewed Papers
15

References

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