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  • F5259 - Anti-Human IgA (α-chain specific)−FITC antibody produced in goat

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F5259 Sigma-Aldrich

Anti-Human IgA (α-chain specific)−FITC antibody produced in goat

affinity isolated antibody, buffered aqueous solution

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Properties

Related Categories Alphabetical Index, Antibodies, FITC Antibodies, HU-HZ, Human IgA Secondary Antibodies and Conjugates,
conjugate   FITC conjugate
clone   polyclonal
biological source   goat
application(s)   direct immunofluorescence: 1:16
form   buffered aqueous solution
storage temp.   2-8°C
antibody form   affinity isolated antibody
Quality Level   200
antibody product type   secondary antibodies

Description

General description

IgA antibody is secretory antibody and is present abundantly in mucous linings of gastrointestinal, respiratory and genitourinary tracts, tears and saliva. Thus secretory IgA contributes to the humoral defense mechanism against the pathogens on mucosal surfaces
Anti-Human IgA (α-chain specific)-FITC antibody is specific for α chain of human IgA. Goat Anti-Human IgA is conjugated to Fluorescein Isothiocyanate (FITC), Isomer I.

Immunogen

Purified human IgA

Application

Anti-Human IgA (α-chain specific)-FITC antibody may be used for direct immunofluorescence at a working antibody dilution of 1:16 to 1:32. A dilution of 1:100 was used for immunohistochemistry of cryosections of human sural nerves.

Physical form

Solution in 0.01 M phosphate buffered saline pH 7.4, containing 1% BSA and 15 mM sodium azide

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Packaging

1, 2 mL in glass bottle

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
WGK 2
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable

Documents

Certificate of Analysis (COA)

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Certificate of Origin (COO)

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Protocols & Articles

Articles

Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy

How to Choose a Secondary Antibody

The following information is provided to help you decide which secondary antibody may be best for your particular application.
Keywords: Amplification, Enzyme-linked immunosorbent assay, Flow cytometry, Immobilization, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Western blot

Secondary Antibodies, Conjugates and Kits

Secondary antibodies are polyclonal or monoclonal antibodies that bind to primary antibodies or antibody fragments, such as the Fc or Fab regions. They are typically labeled with probes that make the...
Keywords: Absorption, Adsorption, Amplification, Bacterial conjugations, Cancer, Digestions, Electrophoresis, Enzyme-linked immunosorbent assay, Flow cytometry, Gel electrophoresis, Gene expression, Hormones, Immobilization, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Immunology, Immunoprecipitation, Infrared spectroscopy, Microbiology, Microscopy, Purification, Vitamins, Western blot

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Peer-Reviewed Papers
15

References

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