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  • F5303 - Monoclonal Anti-Falkor/PHD1 antibody produced in mouse

F5303 Sigma-Aldrich

Monoclonal Anti-Falkor/PHD1 antibody produced in mouse

clone FLK-49, purified immunoglobulin, buffered aqueous solution



Related Categories Alphabetical Index, Angiogenesis, Antibodies, Antibodies for Cell Biology, Antibodies for Cell Stress,
conjugate   unconjugated
clone   FLK-49, monoclonal
biological source   mouse
application(s)   microarray: suitable
  western blot: 0.5 μg/mL using total cell extracts from 293T cells transfected with Falkor-myc tagged
species reactivity   mouse
form   buffered aqueous solution
shipped in   dry ice
storage temp.   −20°C
antibody form   purified immunoglobulin
isotype   IgG2a
Quality Level   200
antibody product type   primary antibodies
UniProt accession no.   Q91YE2
Gene Information   mouse ... Egln2(112406)


General description

Falkor is expressed at different levels in various tissues. It is localized in the nuclei of cells and encoded by 2Kb mRNA. Falkor is ubiquitously expressed in various mouse tissue with high level expression in testis and the lowest expression in spleen and skeletal muscle.

Monoclonal Anti-Falkor/PHD1 (mouse IgG2a isotype) is derived from the FLK-49 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice. The PHD1 gene is mapped to human chromosome 19q13.2and exists in three isoforms.


Monoclonal Anti-Falkor/PHD1 recognizes mouse Falkor/PHD1 (approx. 47 kDa).


synthetic peptide corresponding to amino acids 2-20 of falkor/PHD1.


Monoclonal Anti-Falkor/PHD1 antibody is suitable for microarray and western blot at a concentration of 0.5μg/mL using total cell extracts from 293T cells transfected with Falkor-myc tagged.

Monoclonal Anti-Falkor/PHD1 antibody produced in mouse may be used in enzyme-linked immunosorbent assay (ELISA) and immunoblotting.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Storage and Stability

For continuous use, store at 2-8 °C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing is not recommended. Storage in "frost-free" freezers is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.


Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Biochem/physiol Actions

EGLN2 (Egl nine homolog 2), also known as prolyl hydroxylase domain-containing protein 1 (PHD1), functions as an oxygen sensor in cells and mediates oxygen homeostasis. It inhibits nuclear factor kappa-light-chain-enhancer of activated B-cells (NF-κB) signaling. Polymorphisms in the EGLN2 is implicated in various colorectal, hepatocellular and non-small cell lung cancer.

Falkor, a novel cell growth regulator, is speculated that the falkor protein is associated with suppression of cell growth and comprises of several putative phosphorylation sites for proteins such as protein kinase C and casein kinase II. Falkor has a significant homology to the C.elegans EGL-9. The human homolog of Falkor and EGL-9 is PHD1 (prolyl hydroxylase 1) which is involved in the regulation of HIF-1α. PHD1 function as dioxygenases that regulate HIF-1α by prolyl hydroxylation.

Safety & Documentation

Safety Information

WGK Germany 


Certificate of Analysis (COA)

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Protocols & Articles


Antibody Basics

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