• USA Home
  • F5662 - Monoclonal Anti-HLA Class I Antigen−FITC antibody produced in mouse

F5662 Sigma

Monoclonal Anti-HLA Class I Antigen−FITC antibody produced in mouse

clone W6/32, purified immunoglobulin, buffered aqueous solution

Synonym: Monoclonal Anti-HLA Class I Antigen



Related Categories Alphabetical Index, Analytical Cytology, Antibodies, Antibodies for Cell Biology, Antibodies for Hematopoietic Stem Cells,
species reactivity   human, monkey
application(s)   direct immunofluorescence: 1:10 using acetone-fixed human tonsil frozen sections.
  flow cytometry: 10 μL using 1 × 106 cells
clone   W6/32, monoclonal
antibody form   purified immunoglobulin
form   buffered aqueous solution
isotype   IgG2a
shipped in   wet ice
storage temp.   2-8°C
Gene Information   human ... HLA-A(3105), HLA-B(3106), HLA-C(3107)
biological source   mouse
conjugate   FITC conjugate



human tonsil leukocyte membrane preparation.

General description

The function of HLA class I molecules is to present antigen to CD8+ lymphocytes. They are involved in the regulation of positive and negative selection of CD8+ T cells by thymic epithelial cells during T cell maturation. These molecules are important in allograft rejection and in the linkage of certain haplotypes in autoimmune diseases. Expression of HLA class I antigens may be modulated in pathological states. Malignant cells may lose these antigens while hepatocytes in alcoholic hepatitis, biliary cirrhosis and chronic active hepatitis may display enhanced reactivity. Increased expression of these antigens is described in muscle cells affected by muscular dystrophy, inflammatory myopathy and other neuro-muscular disorders.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide


Recognizes the human HLA Class I antigen expressed on most human nucleated cells. The antibody reacts with a monomorphic epitope on the 44-45 kDa α-chain polypeptide of human class I HLA molecules (HLA-A,B,C). The antibody may be used in immunoprecipitation, complement mediated cytotoxicity, flow cytometry and immunohistochemical staining of acetone-fixed, frozen sections and cell smears. It cross-reacts with cells from African and Asian apes and old world monkeys. No reactivity is seen with cells from most new world monkeys or from non-primates. The epitope recognized by this antibody is sensitive to routine formalin fixation and paraffin embedding.
4th Workshop: code no. P1
5th Workshop: code nos. BP166, BP288, BP407, B 005

Preparation Note

Prepared by conjugation to fluorescein isothiocyanate isomer I (FITC). This green dye is efficiently excited at 495 nm and emits at 525 nm.


FITC Monoclonal Anti-Human HLA Class I Antigen may be used for immunochemical analysis of HLA Class I antigens; Studies of HLA-A,B,C expression on embryonic and neoplastic tissues; and/or the purification of HLA-A,B,C antigens.

Features and Benefits

Antibody Bioguarantee
Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more.

Price and Availability

Antibody Explorer

Explore with Confidence

All labs need water
Safety & Documentation

Safety Information

Personal Protective Equipment  Eyeshields, Gloves, half-mask respirator (US), multi-purpose combination respirator cartridge (US)
RIDADR  NONH for all modes of transport
WGK Germany  nwg
Protocols & Articles

Related Content

Antibody Explorer | Buy Primary & Secondary Antibodies

Monoclonal and polyclonal primary antibodies are focused on cell biology, neurobiology and molecular biology. Secondary antibodies targeting multiple host’s IgG are conjugated to alkaline phosphatase...
Keywords: Amplification, Buffers, Cell biology, Enzyme-linked immunosorbent assay, Immunofluorescence, Immunohistochemistry, Inflammation, Molecular biology, Phosphorylations, Purification, Western blot

Peer-Reviewed Papers


Related Products

related product

Product #


Add to Cart

08168 Timestrip Plus 8 °C

Technical Service:

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Bulk Ordering & Pricing:

Need larger quantities for your development, manufacturing or research applications?