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F5803 Sigma-Aldrich

o-Dianisidine dihydrochloride

Suitable for use in glucose determination

Synonym: 3,3′-Dimethoxybenzidine dihydrochloride, Fast Blue B

  • CAS Number 20325-40-0

  • Empirical Formula (Hill Notation) C14H16N2O2 · 2HCl

  • Molecular Weight 317.21

  •  Beilstein Registry Number 3917996

  •  EC Number 243-737-5

  •  MDL number MFCD00012488

  •  PubChem Substance ID 24894894

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Properties

Related Categories Cell Biology, D, Hematology and Histology, Stains & Dyes, A to Z, Stains and Dyes More...
InChI Key   UXTIAFYTYOEQHV-UHFFFAOYSA-N
storage temp.   2-8°C

Description

General description

A preweighed vial containing 50 mg o-Dianisidine.

Specificity

o-Dianisidine (3,3′-dimethoxybenzidine) is a peroxidase substrate suitable for use in ELISA procedures. This substrate produces a soluble end product that is yellow-orange in color and can be read spectrophotometrically at 405 nm. The reaction may be stopped with 5 M HCl.

Warning

Probably carcinogenic.

Application

o-Dianisidine dihydrochloride is suitable for use in the glucose determination. It is suitable reagent used in the following studies:
• As substrate for the determination of serum ceruloplasmin from its oxidase activity.
• Determination of total antioxidant response (TAR) against potent free radical reactions by a novel, colorimetric and fully automated method.
• As colorimetric indicator for the simultaneous semi-quantitative estimation of glucose, lactate and uric acid on paper-based microfluidic devices (microPAD).
• Myeloperoxidase activity assay.

Safety & Documentation

Safety Information

Symbol 
Signal word 
Danger
Hazard statements 
Precautionary statements 
RIDADR 
NONH for all modes of transport
WGK Germany 
3
RTECS 
DD1050000

Milli-Q® Water Purification Solutions
Protocols & Articles

Protocols

Enzymatic Assay of Diamine Oxidase (E.C. No. 1.4.3.22)

Replacing OP SPPUTR01. New document number assigned to conform to current document numbering system.
Keywords: Carcinogens, Extinction coefficient

Enzymatic Assay of Glucose Oxidase

Derived from procedure SPGLUC01. Includes template updates to current SOP specification and incorporation of notes into the procedure. Refer to CR SOP-DEK ENZ 36.
Keywords: Extinction coefficient

Peer-Reviewed Papers
15

References

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