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F5884 Sigma-Aldrich

α-L-Fucosidase from bovine kidney

ammonium sulfate suspension, ≥2.0 units/mg protein (biuret)

Synonym: α-L-Fucoside fucohydrolase

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Properties

Related Categories 3.2.x.x Glycosidases, 3.x.x.x Hydrolases, Application Index, Biochemicals and Reagents, Carbohydrate Hydrolysis,
form   ammonium sulfate suspension
foreign activity   α-mannosidase and β-galactosidase ≤0.1%
  β-N-acetylglucosaminidase ≤0.2%
shipped in   wet ice
storage temp.   2-8°C

Description

General description

α-L-fucosidases is a liposomal enzyme, which belongs to glycosyl hydrolases family.

Application

α-L-fucosidase from bovine kidney has been used:
• to study their ability to alter primary cell transduction
• to examine the role of fucose residues of cell surface glycans in adhesion
• to investigate its role during fertilization

Biochem/physiol Actions

α-L-fucosidases degrades fucose-containing fucoglycoconjugates and breaks fucosidic bonds in oligosaccharides and glycoconjugates. It acts as a tumor marker for hepatic and colorectal cancer. α-L-fucosidase exhibits transglycosylation property.

Unit Definition

One unit will hydrolyze 1.0 μmole of p-nitrophenyl α-L-fucoside to p-nitrophenol and L-fucose per min at pH 5.5 at 25°C.

Physical form

Suspension in 3.2 M (NH4)2SO4, 10 mM NaH2PO4 10 mM citrate, pH 6.0

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
1

Documents

Certificate of Analysis (COA)

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Certificate of Origin (COO)

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Protocols & Articles

Articles

Ammonium Sulfate Suspensions - Technical Note

Note that for an ammonium sulfate suspension, most of the enzyme will be in solid form. It is likely that only negligible amounts of enzyme will be in the ammonium sulfate solution.
Keywords: Enzyme activity

Related Content

Enzymes & Proteins

Application Index | Enzyme Index | Substrate Index | Inhibitor Index | Cofactor Index | Lectin Index
Keywords: Cell signaling, Diagnostic, Drug discovery, Metabolomics, Molecular biology

Peer-Reviewed Papers
15

References

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