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F6258 Sigma-Aldrich

Anti-Rat IgG (whole molecule)–FITC antibody produced in goat

affinity isolated antibody, buffered aqueous solution

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Properties

Related Categories Alphabetical Index, Antibodies, FITC Antibodies, Primary Antibodies, RAE-RAX,
conjugate   FITC conjugate
clone   polyclonal
biological source   goat
application(s)   direct immunofluorescence: 1:32
form   buffered aqueous solution
storage temp.   2-8°C
antibody form   affinity isolated antibody
Quality Level   200
antibody product type   secondary antibodies

Description

General description

An immunoglobulin has two heavy chain and two light chain connected by disulfide bond. It mainly helps in immune defense. It is a glycoprotein. IgG is a major class of immunoglobulin. Immunoglobulin G (IgG) participates in hypersensitivity type II and type III. Rat has five immunoglobulin classes- IgM, IgG, IgA, IgD and IgGE. IgG has four subclasses- IgG1, IgG2a, IgG2b and IgG2c. It helps in opsonization, complement fixation and antibody dependent cell mediated cytotoxicity.

IgG antibody subtype is the most abundant serum immunoglobulins of the immune system. It is secreted by B cells and is found in blood and extracellular fluids and provides protection from infections caused by bacteria, fungi and viruses. Maternal IgG is transferred to fetus through the placenta that is vital for immune defence of the neonate against infections. Anti-Rat IgG (whole molecule)-FITC antibody is specific for all rat IgG subclasses. Goat anti-rat IgG is purified by affinity isolation and then conjugated to FITC.

IgG is the main antibody type found in plasma and extracellular fluid and is expressed on B cell membrane. It regulates several functions such as complement activation and phagocytosis and hence plays a pivotal role in facilitating cytological immune responses. Anti-rat IgG (whole molecule)-FITC antibody can be used as a secondary antibody in indirect immunofluorescence. Goat anti- rat IgG (whole molecule)-FITC antibody reacts specifically with all rat immunoglobulins.

Immunogen

Purified rat IgG.

Purified rat IgG

Application

Anti-Rat IgG (whole molecule)-FITC antibody may be used for immunofluorescent labeling of rat spleen cells. For immunofluorescence of rat cartilage tissue sections, a concentration of 5-10 μg/ml was used. The range of dilutions of anti-rat IgG used for immunofluorescence is between 1:10 and 1:250 in various studies.. The antibody was also used in flow cytometry applications.

Anti-Rat IgG (whole molecule)−FITC antibody has been used in immunofluorescence measurement of antibody-treated cells and staining of monolayers.

Anti-rat IgG (whole molecule)-FITC antibody (diluted 1:20 in PBS) can be used as a secondary antibody in immunolabelling. It can also be used in flow cytometry.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Packaging

0.5 mL in glass insert

1, 2 mL in glass bottle

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable

Documents

Certificate of Analysis (COA)

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Certificate of Origin (COO)

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Protocols & Articles

Articles

Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
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The following information is provided to help you decide which secondary antibody may be best for your particular application.
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Secondary Antibodies, Conjugates and Kits

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Peer-Reviewed Papers
15

References

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