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  • F6550 - Anti-FLIPα, C-Terminal antibody produced in rabbit

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F6550 Sigma-Aldrich

Anti-FLIPα, C-Terminal antibody produced in rabbit

IgG fraction of antiserum, buffered aqueous solution

Synonym: Anti-CASH, Anti-CLARP, Anti-Casper, Anti-FLAME-1, Anti-I-FLICE

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Properties

Related Categories Alphabetical Index, Antibodies, Antibodies for Apoptosis, Antibodies for Cell Biology, Antibodies to Suppressors of Apoptosis,
conjugate   unconjugated
clone   polyclonal
biological source   rabbit
application(s)   western blot: 0.5 μg/mL using human HeLa cell extract
species reactivity   human
mol wt   antigen 55 kDa
form   buffered aqueous solution
shipped in   dry ice
storage temp.   −20°C
antibody form   IgG fraction of antiserum
Quality Level   200
antibody product type   primary antibodies
UniProt accession no.   O15519
Gene Information   human ... CFLAR(8837)

Description

Specificity

By immunoblotting, the antibody detects human FLIPα (FLICE-inhibitory protein).

Immunogen

synthetic peptide corresponding to amino acids 447-464 of human FLIPα/FLIPL.

Application

Anti-FLIPα, C-Terminal antibody is suitable for:
• western blotting of whole L540 cell extracts to detect c-flip activity in the regulation of NF-κB distribution and activity by 5F11 and bortezomib
• immunohistochemistry analysis to study the optimization of c-FLIP activity in lymphoma cells
• immunohistochemical cytoplasmic c-Flip staining to detect c-FLIP expression in Burkitt′s lymphoma (BL) may be a reliable prognostic marker
• western blot analysis at a concentration of 0.5μg/mL using human HeLa cell extract.

Physical form

Solution in phosphate buffered saline containing 0.02% sodium azide

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety & Documentation

Safety Information

WGK Germany 
WGK 2
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable

Documents

Certificate of Analysis (COA)

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Certificate of Origin (COO)

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Protocols & Articles

Articles

Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy

Protocols

Western Blot Protocol | Immunoblotting Protocol

Western Blotting refers to the electrophoretic transfer of proteins from sodium dodecyl sulfate polyacrylamide gels to sheets of PVDF or nitrocellullose membrane, followed by immunodetection of prote...
Keywords: AGE, Buffers, Cell disruption, Detection methods, Detergents, Dialysis, Electroblotting, Electrophoresis, Enzyme activity, Gel electrophoresis, Immunoprecipitation, PAGE, Protein extraction, Purification, Sample preparations, Western blot

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Peer-Reviewed Papers
15

References

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