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F7367 Sigma-Aldrich

Anti-Goat IgG (whole molecule)–FITC antibody produced in rabbit

affinity isolated antibody, buffered aqueous solution

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Properties

Related Categories Alphabetical Index, Antibodies, Antibody-Fluorochrome Conjugates, FITC Antibodies, GM-GO,
conjugate   FITC conjugate
clone   polyclonal
biological source   rabbit
application(s)   direct immunofluorescence: 1:400
  immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:400
form   buffered aqueous solution
storage temp.   −20°C
antibody form   affinity isolated antibody
Quality Level   200
antibody product type   secondary antibodies
storage condition   protect from light

Description

General description

IgG antibodies are smallest and are found in all body fluids and capable of fighting bacterial and viral infection. It is the only type of antibody able to cross the placenta and immunise the foetus. The anti-goat IgG (whole molecule)-FITC antibody is useful in indirect immunofluorescence studies of immunised goat sera infected Trichostrongylus colubriformis (nematode). Antigens can be measured by quantitative immunofluorescence using image analysis, in tissue sections stained with FITC labelled rabbit anti goat IgG. Anti-goat IgG antibody reacts specifically with all goat immunoglobulins.

IgG is a major class of immunoglobulin. Goat IgG has two subclasses- IgG1 and IgG2.

Immunoglobulin G (IgG) is found in blood and has four polypeptide chains. It contains two identical 25 kDa κ or λ light (L) chains and two identical 50 kDa γ heavy (H) chains. The light and heavy chains are interconnected by disulfide bonds. It is the most important type of glycoprotein antibody present in human serum, secreted by the B cells. IgG exists in four isotypes: IgG1, IgG2, IgG3, and IgG4. IgG has the longest serum half-life compared to other immunoglobulins.

Immunogen

IgG purified from Goat

Application

Anti-Goat IgG (whole molecule)−FITC antibody has been used:
• in double labeling for laminin and α-fetoprotein (AFP) at a dilution of 1:400
• in immunohistochemistry at a dilution of 1:50
• in immunocytochemistry
• in immunochemical jagged-1 analyses
• in immunofluorescence at a dilution of 1:200

Anti-goat IgG antibody can be used in antisera localization for neurofilament antigens obtained in rat peripheral nerve and can be done in tissues of rat and human peripheral and central nervous system by the aid of indirect immunofluorescence.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Storage and Stability

For continuous use, store at 2-8 °C for up to one month. For extended storage, the solution may be frozen in working aliquots. Repeated freezing and thawing is not recommended. Storage in "frost-free" freezers not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Biochem/physiol Actions

Immunoglobulin G (IgG) participates in hypersensitivity type II and type III.

Immunoglobulin G (IgG) stimulates the classical pathway of the complement system. Maternal IgG is transferred to the fetus through the placenta. This is vital for the immune defense of the neonate against infections. IgG regulates antibody-dependent cell-mediated cytotoxicity (ADCC) and might be associated with allergy. It nullifies virus particles and toxins. Digestion of IgG by papain produces fragment antigen-binding (Fab). Pepsin digestion of IgG generates crystallizable (fc) fragment and a single dimeric F(ab)2. IgG levels in severe acute respiratory syndrome coronavirus (SARS-CoV)-2 infected patients may be used as a possible tool to assess the severity and prognosis of coronavirus disease 2019 (COVID-19).

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
nwg
Protocols & Articles

Articles

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Peer-Reviewed Papers
15

References

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