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F7378 Sigma-Aldrich

Fluorescein diacetate

used as cell viability stain

Synonym: 3,6-Diacetoxyfluoran, Di-O-acetylfluorescein

  • CAS Number 596-09-8

  • Empirical Formula (Hill Notation) C24H16O7

  • Molecular Weight 416.38

  •  Beilstein/REAXYS Number 62575

  •  EC Number 209-877-6

  •  MDL number MFCD00005062

  •  PubChem Substance ID 24894939

  •  NACRES NA.47



Related Categories Alphabetical, Biochemicals and Reagents, Cell Biology, E-F, Fluorescent Indicators and Probes,
Quality Level   200
form   powder
application(s)   hematology: suitable
  histology: suitable
mp   200-203 °C (lit.)
solubility   acetone: 25 mg/mL
ε (extinction coefficient)   ≥86000 at 488-494 nm in 0.1 M NaOH
Featured Industry   Diagnostic Assay Manufacturing
storage temp.   room temp
SMILES string   CC(=O)Oc1ccc2c(Oc3cc(OC(C)=O)ccc3C24OC(=O)c5ccccc45)c1
InChI   1S/C24H16O7/c1-13(25)28-15-7-9-19-21(11-15)30-22-12-16(29-14(2)26)8-10-20(22)24(19)18-6-4-3-5-17(18)23(27)31-24/h3-12H,1-2H3


General description

Fluorescein diacetate (FDA) is a non-fluorescent molecule, which is hydrolysed to fluorescent fluorescein in live cells. Dead cells cannot accumulate or hydrolyse FDA.


Fluorescein diacetate has been used for the differentiation of live cells from dead cells.


5, 10, 25, 100 g in glass bottle


Lipase substrate

Safety & Documentation

Safety Information

Personal Protective Equipment 
NONH for all modes of transport
WGK Germany 
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable


Certificate of Analysis (COA)

Please Enter a Lot Number

Certificate of Origin (COO)

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Frequently Asked Questions

Which document(s) contains shelf-life or expiration date information for a given product?
If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.
How do I get lot-specific information or a Certificate of Analysis?
The lot specific COA document can be found by entering the lot number above under the "Documents" section.
Is Product F7378, Fluorescein diacetate, a fluorescent compound?
No. Only upon hydrolysis of the esters, catalyzed by cellular esterase, the lactone ring opens yielding fluorescein.Only the fluorescein form is fluorescent.
After Product F7378, Fluorescein diacetate, has been converted to fluorescein, what are the maximum excitation and emission wavelengths?
The maximum excitation wavelength is 490 nm and the maximum emission wavelength is 526 nm.
What are some common applications for Product F7378, Fluorescein diacetate?
This compound is used extensively for the staining of living cells.It has been used as a viability stain for cultured neurons and plant ways, and in measuring of esterase and cholinesterase activity.See the following references for usage:Fluorometric determination of lipase: Guilbault, G.G. and Kramer, D.N., "Fluorometric Determination of Lipase, Acylase, Alpha- and Gamma- Chymotrypsin and Inhibitors of These Enzymes", Anal. Chem. 36, 409, 1964. ; Anal. Biochem., Vol. 14, 28 (1966);Fluorescent stain for viable rosette-forming cells: J. Immun. Meth., Vol. 5,305 (1974);Fluorogenic substrate detection of viable pathogenic protozoa: Science, Vol. 227, 435 (1985);Reference Standard in antibody studies: Anal. Biochem., Vol. 9, 474 (1964);Cell viability staining: Staining Procedures, G. Clark, 4th ed., p. 93.Guilbault, G.G. and Kramer, D.N., "Fluorometric Determination of Lipase, Acylase, Alpha- and Gamma- Chymotrypsin and Inhibitors of These Enzymes", Anal. Chem. 36, 409, 1964.
What solubility information do you have on Product F7378, Fluorescein diacetate?
We have found that it is slightly soluble in water at 0.1%, and in ethanol at 0.3%. It is also soluble in dimethylformamide (DMF).
How stable are solutions ofProduct F7378, Fluorescein diacetate, once prepared?
Based on our experience, stock solutions of fluorescein diacetate at 5 mg/mL in acetone or DMSO can be stored at -20 °C. According to one on-line source, solutions at 5 mg/mL in acetone or ethanol can be stored refrigerated.
How do I find price and availability?
There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote. USA customers: 1-800-325-3010 or view local office numbers.
What is the Department of Transportation shipping information for this product?
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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