• USA Home
  • F7387 - Monoclonal Anti-Fibronectin antibody produced in mouse

EMAIL THIS PAGE TO A FRIEND
F7387 Sigma-Aldrich

Monoclonal Anti-Fibronectin antibody produced in mouse

clone FN-15, ascites fluid

Purchase

Properties

Related Categories Alphabetical Index, Antibodies, Antibodies for Cell Biology, Antibodies for Hematopoietic Stem Cells, Antibodies for Stem Cell Biology,
conjugate   unconjugated
clone   FN-15, monoclonal
biological source   mouse
application(s)   indirect ELISA: 1:5,000
  indirect immunofluorescence: 1:200 using cultured human fibroblasts
  microarray: suitable
  western blot: 1:800
species reactivity   mouse, human, chicken
shipped in   dry ice
storage temp.   −20°C
antibody form   ascites fluid
isotype   IgG1
Quality Level   200
antibody product type   primary antibodies
contains   15 mM sodium azide
UniProt accession no.   P02751
Gene Information   human ... FN1(2335)
mouse ... Fn1(14268)

Description

General description

Monoclonal Anti-Fibronectin (mouse IgG1 isotype) is derived from the hybridoma produced the fusion of mouse myeloma cells and splenocytes from immunized mice. Fibronectin (FN) belongs to glycoprotein family widely expressed in many tissues. FN is an extracellular matrix protein composed of two nearly-identical disulfide-bound polypeptides with typical molecular weights of 220-240 kDa. There are three isoforms of fibronectin, that includes cellular FN, plasma FN and fetal FN. Cellular fibronectin is structurally and antigenically similar to cold insoluble globulin from plasma and antibodies to either form usually cross-react. Careful analysis of the FN molecule indicates that it contains several functionally and structurally distinct domains which may bind to cell surfaces and to a variety of molecules such as collagen, heparin, gelatin, fibrin and DNA. FN gene in human chromosome is mapped to 2q35.

Mouse monoclonal clone FN-15 anti-Fibronectin antibody labels fibronectin in the pericellular extracellular matrix of cultured human fibroblasts using indirect immunofluorescent methods. It also labels fibronectin in chicken and mouse cells. The FN-15 antibody specifically stains fibronectin in immunoblotting, and in an ELISA is reactive with human plasma fibronectin.

Immunogen

fibronectin from human plasma

Application

Monoclonal Anti-Fibronectin may be used to specifically localize fibronectin in human cell culture and in tissue sections by immunoblotting, immunohistochemical techniques and to stain fibronectin in hydrogels.

Mouse monoclonal clone FN-15 anti-Fibronectin antibody may be used to specifically localize fibronectin in human cell culture and in tissue sections by immunoblotting and immunohistochemical techniques. Monoclonal antibodies reacting specifically with FN may be used to localize FN in human cell cultures, in tissue sections and in plasma.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Biochem/physiol Actions

Fibronectin (FN) play an important role in diverse biological phenomena including cell adhesion, cell migration, hemostasis and thrombosis, wound healing and the ability to induce a more normal phenotype in transformed cells. FN is upregulated in many cancers. FN increases the expression of matrix metalloproteinases (MMPs), promoting cancer cell invasion and metastasis.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
WGK 2
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable

Documents

Certificate of Analysis (COA)

Please Enter a Lot Number

Certificate of Origin (COO)

Please Enter a Lot Number
Protocols & Articles

Articles

Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy

Protocols

Western Blot Protocol | Immunoblotting Protocol

Western Blotting refers to the electrophoretic transfer of proteins from sodium dodecyl sulfate polyacrylamide gels to sheets of PVDF or nitrocellullose membrane, followed by immunodetection of prote...
Keywords: AGE, Buffers, Cell disruption, Detection methods, Detergents, Dialysis, Electroblotting, Electrophoresis, Enzyme activity, Gel electrophoresis, Immunoprecipitation, PAGE, Protein extraction, Purification, Sample preparations, Western blot

Related Content

Antibody Explorer | Buy Primary Antibodies & Secondary Antibodies

Primary, Secondary and Recombinant Monoclonal Antibodies Providing highly cited primary and secondary antibodies, we have you covered for your ELISA, western blot, immunohistochemistry or other assay...
Keywords: Alzheimer Disease, Cancer, Chromatin immunoprecipitation, Diagnostic, Enzyme-linked immunosorbent assay, Flow cytometry, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Neuroscience, Western blot

Peer-Reviewed Papers
15

References

Related Products

Technical Service:

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Bulk Ordering & Pricing:

Need larger quantities for your development, manufacturing or research applications?