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F7425 Millipore

ANTI-FLAG® antibody produced in rabbit

affinity isolated antibody, buffered aqueous solution

Synonym: Anti-ddddk, Anti-dykddddk

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Properties

Related Categories Alphabetical Index, Antibodies, Antibodies and Conjugates, Antibodies to Peptide and Protein Fusion Tags, FK-FO,
conjugate   unconjugated
clone   polyclonal
biological source   rabbit
application(s)   dot blot: 1-2.5 μg/mL
  immunoprecipitation (IP): 4-8 μg using amino terminal FLAG-BAP fusion protein from E. coli crude lysate
  indirect immunofluorescence: 5-10 μg/mL using 293T cells transfected with a plasmid encoding FLAG-JNK
  western blot (chemiluminescent): 1-2.5 μg/mL using an E. coli periplasmic extract expressing an N-terminal FLAG fusion protein
species reactivity   all
form   buffered aqueous solution
shipped in   dry ice
storage temp.   −20°C
antibody form   affinity isolated antibody
isotype   IgG
antibody product type   primary antibodies
concentration   ~0.8 mg/mL
immunogen sequence   DYKDDDDK

Description

General description

The FLAG epitope, is an eight amino acid protein with an enterokinase-cleavage site.

The rabbit Anti-FLAG polyclonal affinity antibody ANTI-FLAG recognizes the FLAG epitope located on FLAG fusion proteins. This antibody reacts with N-terminal, N-terminal-Met, and C-terminal FLAG fusion proteins.

Immunogen

FLAG; peptide sequence DYKDDDDK

Application

The antibody recognizes the FLAG epitope located on FLAG-tagged fusion proteins at the N-terminus or C-terminus, applying dot blot, immunoblotting, immunoprecipitation and immunocytochemistry assays.
Browse additional application references in our FLAG® Literature portal."

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide

Legal Information

FLAG is a registered trademark of Sigma-Aldrich Co. LLC

Biochem/physiol Actions

FLAG epitope plays a crucial role in immunoaffinity purification of fusion proteins.

Preparation Note

Purified by affinity chromatography on a column bearing the immunizing peptide.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
2

Documents

Certificate of Analysis

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Certificate of Origin

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Frequently Asked Questions

Is Product F7425, ANTI-FLAG® from rabbit, a monoclonal or polyclonal?
F7425 is a polyclonal antibody from rabbit.
What is the concentration of Product F7425, ANTI-FLAG® from rabbit?
The concentration is 0.9 mg protein per ml.
How much Product F7425, ANTI-FLAG® from rabbit, should be used in western blotting and ELISA applications?
For a Western blot from a PAGE run (preferably) under denaturing conditions, a 1:400 dilution of this antibody should be used.A 1-10 μg/ml concentration should be used when using thie antibody in an ELISA application.
How to get rid of background when using Product F7465, ANTI-FLAG® from rabbit,?
To reduce non-specific background on tissue staining, a small amount of Triton X-100 (Product No. X100 or T8787), can be added to the wash buffers and antibody incubation buffers. Try a Triton X-100 concentration of 0.1% to start. Tween 20 (Product No. P5927) can also be used.
Is there any references for the use of Product F7465, ANTI-FLAG® from rabbit, in immunohistochemistry or western blotting?
K. Schafer and T. Braun, Monoclonal Anti-FLAG Antibodies React with a New Isoform of Rat Mg++ Dependent Protein Phosphatase. Biochemical and Biophysical Research Communications, 207, 708.
What are the positive controls that can be used with Product F7425, ANTI-FLAG® from rabbit?
The positive controls that can be used are as follows:Amino-terminal FLAG-BAP fusion protein, Product No. P7582Carboxy-terminal FLAG-BAP fusion protein, Product No. P7457Amino-terminal Met-FLAG-BAP fusion protein, Product No. P5975.
Which document(s) contains shelf-life or expiration date information for a given product?
If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.
How do I get lot-specific information or a Certificate of Analysis?
The lot specific COA document can be found by entering the lot number above under the "Documents" section.
How do I find price and availability?
There are several ways to find pricing and availability for our products.Once you log onto our website, you will find the price and availability displayed on the product detail page.You can contact any of our Customer Sales and Service offices to receive a quote. USA customers: 1-800-325-3010 orview local office numbers.
What is the Department of Transportation shipping information for this product?
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
My question is not addressed here, how can I contact Technical Service for assistance?
Ask a Scientist here.
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Protocols & Articles

Articles

Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
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Peer-Reviewed Papers
15

References

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