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F7776 Sigma-Aldrich

Monoclonal Anti-MAP Kinase, Activated (Diphosphorylated ERK-1&2)−FITC antibody produced in mouse

clone MAPK-YT, purified immunoglobulin, buffered aqueous solution

Synonym: Monoclonal Anti-MAP Kinase, Activated (Diphosphorylated ERK-1&2)

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Properties

Related Categories Alphabetical Index, Antibodies, Antibodies for Cell Biology, Antibodies for Hematopoietic Stem Cells, Antibodies for Kinase/Phosphatase Biology,
conjugate   FITC conjugate
clone   MAPK-YT, monoclonal
biological source   mouse
application(s)   direct immunofluorescence: 1:20 using cultured human foreskin fibroblasts (paraformaldehyde-fixation)
species reactivity   mouse, Xenopus, Drosophila, Caenorhabditis elegans, rat, yeast, hamster, bovine, human
mol wt   antigen ERK-1 44 kDa
  antigen ERK-2 42 kDa
form   buffered aqueous solution
shipped in   dry ice
storage temp.   −20°C
antibody form   purified immunoglobulin
isotype   IgG1
antibody product type   primary antibodies
UniProt accession no.   P28482
Gene Information   human ... MAPK1(5594), MAPK3(5595)
mouse ... Mapk1(26413), Mapk3(26417)
rat ... Mapk1(116590), Mapk3(50689)

Description

Specificity

The antibody reacts specifically with the diphosphorylated form of MAP kinase (ERK-1 and ERK-2). It does not recognize the non-phosphorylated or the monophosphorylated forms of MAP kinase or the diphosphorylated forms of JNK and p38 MAP kinase. The epitope recognized by the antibody contains the phosphorylated threonine and tyrosine residues within the regulatory site of active MAP kinase.

Immunogen

synthetic peptide HTGFLpTEpYVAT corresponding to the phosphorylated form of the ERK-activation loop.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
nwg

Documents

Certificate of Analysis (COA)

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Protocols & Articles

Articles

Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy

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Peer-Reviewed Papers
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References

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