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  • F8051 - Anti-phospho-FAK (pTyr407) antibody produced in rabbit

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F8051 Sigma

Anti-phospho-FAK (pTyr407) antibody produced in rabbit

affinity isolated antibody, buffered aqueous solution

Synonym: Anti-phospho-Focal Adhesion Kinase

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Properties

Related Categories Alphabetical Index, Antibodies, Antibodies for Cell Biology, Antibodies for Kinase/Phosphatase Biology, Antibodies to Phosphoproteins at Phosphotyrosine (pTyr),
species reactivity   mouse, human, rat, frog, chicken
application(s)   western blot: suitable
clone   polyclonal
antibody form   affinity isolated antibody
form   buffered aqueous solution
mol wt   antigen mol wt 125 kDa
shipped in   dry ice
storage temp.   −20°C
Gene Information   human ... PTK2(5747)
mouse ... Ptk2(14083)
rat ... Ptk2(25614)
biological source   rabbit
conjugate   unconjugated

Description

Immunogen

synthetic phosphopeptide derived from the region of FAK that contains tyrosine 407.

General description

Focal Adhesion Kinase (FAK) is a non-receptor protein tyrosine kinase that is expressed ubiquitously. FAK is the critical in forming cell-substratum adhesions or focal adhesion complexes. FAK reportedly has multiple phosphorylation sites. Autophosphorylation of FAK at Tyr397 creates a binding site on FAK for Src-family kinases and is critical for maximum adhesion and migration responses. The phosphorylation of Tyr407 and Tyr861 is induced during epithelial-mesenchymal transition and further augmented during cell migration. FAK activation couples signal transduction via PI3K, CAS and Src. FAK signaling is involved in multiple functions such as proliferation, migration, survival and angiogenesis. Disruption of FAK signalling results in failure of adhesion to the substratum and anoikis (apoptosis) of epithelial cells which are anchorage dependent. FAK has been observed to be upregulated in human tumors such as neuroblastoma and is related to tumor cell survival and prognosis
Anti-phospho-FAK (pTyr407) recognizes FAK (Focal Adhesion Kinase) phosphorylated at tyrosine 407 (125 kDa).

Physical form

Solution in Dulbecco′s phosphate buffered saline (without magnesium and calcium), pH 7.3 (+/- 0.1), 50% glycerol with 1.0 mg/mL bovine serum albumin (IgG, protease free) as a carrier and 0.05% sodium azide as a preservative.

Application

A starting dilution of 1:1000 is suitable for detection of FAK phosphorylated at tyrosine 407 by immunoblotting.

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