• USA Home
  • F8141 - Fibronectin-like Engineered Protein Polymer-plus genetically engineered

EMAIL THIS PAGE TO A FRIEND
F8141 Sigma-Aldrich

Fibronectin-like Engineered Protein Polymer-plus genetically engineered

powder, sterile; autoclaved, BioReagent, suitable for cell culture

Synonym: ProNectin® F Plus

Purchase

Properties

Related Categories Attachment Factors, Attachments Factors, Biochemicals and Reagents, Cell Culture, Core Bioreagents,
sterility   sterile; autoclaved
product line   BioReagent
form   powder
mol wt   mol wt ~110 kDa by SDS-PAGE
packaging   pkg of 1 mg
solubility   H2O: 1 mg/mL (stable for 12 mo. at RT)
suitability   suitable for cell culture
storage temp.   room temp

Description

Application

Recommended for use as a cell culture substratum
at 2-10 μg/cm2.

Other Notes

Positively-charged, protein polymer which incorporates multiple copies of the RGD cell attachment epitope from human fibronectin between repeated structural peptide units.

Linkage

Similar to F5022 but more highly charged polymer which encourages better adhesion in many cell lines.

Reconstitution

Dilutions can be made to a working concentration with balanced salt solution; stable up to 2 days at RT. Coated labware is stable at least 4 months at RT.

Legal Information

ProNectin is a registered trademark of Sanyo Chemical Industries, Ltd

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
3

Documents

Certificate of Analysis

Certificate of Origin


Request a Serum Sample

Cell Culture Guide
Protocols & Articles

Articles

Extracellular Matrix Proteins and tools for cell culture optimization

Animal cells and tissue culture techniques are constantly improved to optimize in vitro cell culture conditions. Extracellular Matrix (ECM) proteins coating, chemical or physical modification of the ...
Keywords: Adhesion, Angiogenesis, Apoptosis, Asymmetric synthesis, Cancer, Cell attachment, Cell culture, Cell proliferation, Coagulation, Endocrinology, Growth factors, Hormones

Fibronectin (FN)

Fibronectin (FN) is a multifunctional, extracellular matrix glycoprotein composed of two nearly identical disulfide bound polypeptides of molecular weight 220 kDa. Cellular fibronectin is structurall...
George Sitterley
BioFiles 2008, 3.8, 8.
Keywords: Adhesion, Cell culture, transformation

What is the Extracellular Matrix?

The extracellular matrix (ECM) is secreted by cells and surrounds them in tissues. It has long been understood to be the structural support for cells since its characteristics set the characteristics...
Keywords: Adhesion, Cell culture, Cellular processes, Epimerizations, Growth factors, Immobilization, Sulfations

Related Content

Cell Culture Troubleshooting Guide

Cell culture has become one of the most fundamental techniques for modeling biological systems, and is of increasing importance in the biotechnology and pharmaceutical sectors as well as an essential...
Keywords: Antibiotics, Apoptosis, Cell attachment, Cell culture, Cryopreservation, Filtration, Growth factors, Pharmaceutical, Phase transitions

Peer-Reviewed Papers
15

References

Related Products

Technical Service:

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Bulk Ordering & Pricing:

Need larger quantities for your development, manufacturing or research applications?