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F8317 Sigma-Aldrich

Fetal Bovine Serum

New Zealand origin, Heat Inactivated, sterile-filtered, suitable for cell culture, suitable for hybridoma

Synonym: FBS, FCS, Fetal Bovine Serum, sera, serum

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Properties

Related Categories Cell Culture, FBS Premier, Fetal Bovine Serum (FBS) and Other Animal Sera, Fetal Bovine Serum (FBS) for cell culture
Quality Level   500
biological source   bovine fetus
sterility   sterile-filtered
composition   Bovine IgG, ≤1 mg/mL
  Hemoglobin, ≤20 mg/dL
origin   New Zealand origin
application(s)   cell culture | hybridoma: suitable
  cell culture | mammalian: suitable
impurities   ≤10 EU/mL endotoxin
shipped in   dry ice
storage temp.   −20°C

Description

General description

FBS remains a popular media supplement because it provides a wide array of functions in cell culture. FBS delivers nutrients, growth and attachment factors and protects cells from oxidative damage and apoptosis by mechanisms that are difficult to reproduce in serum-free media (SFM) systems.

Application

FBS derived from clotted blood is the most widely used undefined supplement in eucaryotic, especially mammalian, cell culture. It is typically used with the classical media that were developed in the 1950s and 1960s. These include many popular media such as Minimum Essential Medium (MEM); Dulbecco′s Modified Eagles Media (DME, DMEM); Iscove′s Modified Eagles Medium (IMDM); M199 and Roswell Park Memorial Institute medium, RPMI-1640.

Fetal Bovine Serum has been used in Dulbecco′s Modified Eagle Medium (DMEM) media for primary cell line generation.

Linkage

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Preparation Note

Prepared by heating at 56°C for 30 minutes.

This product is prepared by heating at 56°C for 30 minutes. Heating serum to 56°C for 30 minutes inactivates the complement system for immunoassays. Heat activation also inactivates other undetermined inhibitors of cell growth in culture.

Analysis Note

• Endotoxin and hemoglobin tested

• Tested for the presence of bacteria, virus, and mycoplasma
• Triple filtered with 0.1 micron membrane under aseptic conditions

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
WGK 3
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable

Documents

Certificate of Analysis (COA)

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Protocols & Articles

Articles

Serum for Cell and Tissue Culture: Frequently Asked Questions (FAQs)

Serum is added as a supplement to culture media at a concentration of 2-10% to provide a mixture of nutritional, hormonal, growth and attachment factors. Serum also acts as a buffer to the cell cultu...
Keywords: Agriculture, Cell culture, Centrifugation, Culture media, Filtration, Nucleic acid denaturation

Related Content

Cell Culture Troubleshooting Guide

Cell culture has become one of the most fundamental techniques for modeling biological systems, and is of increasing importance in the biotechnology and pharmaceutical sectors as well as an essential...
Keywords: Antibiotics, Apoptosis, Cell attachment, Cell culture, Cryopreservation, Filtration, Growth factors, Pharmaceutical, Phase transitions

Peer-Reviewed Papers
15

References

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