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F8521 Sigma-Aldrich

Anti-Mouse IgG (Fab specific) F(ab′)2 fragment–FITC antibody produced in goat

affinity isolated antibody, buffered aqueous solution

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Properties

Related Categories Alphabetical Index, Antibodies, FITC Antibodies, MO-MR, Mouse Secondary Antibodies and Conjugates,
conjugate   FITC conjugate
clone   polyclonal
biological source   goat
application(s)   immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:320
form   buffered aqueous solution
storage temp.   −20°C
antibody form   affinity isolated antibody
Quality Level   200
antibody product type   secondary antibodies
storage condition   protect from light

Description

General description

IgG antibody subtype is the most abundant serum immunoglobulins of the immune system. It is secreted by B cells and is found in blood and extracellular fluids and provides protection from infections caused by bacteria, fungi and viruses. Maternal IgG is transferred to fetus through the placenta that is vital for immune defence of the neonate against infections.

Immunoglobulin G (IgG) is a member of the immunoglobulin family and contains gamma (γ) heavy chain in the constant (C) region. Monomeric structure of IgG contains two identical heavy chains and two identical light chains with molecular weight of 50 kDa and 25 kDa respectively. The primary structure of this antibody also contains disulfide bonds implicated in linking the two heavy chains, linking the heavy and light chains and resides inside the chains. IgG is further subdivided into four classes namely, IgG1, IgG2, IgG3 and IgG4 with different heavy chains, named γ1, γ2, γ3 and γ4, respectively.

Specificity

Binds all mouse Igs.
Useful when trying to avoid background staining due to the presence of Fc receptors. Anti-Mouse IgG (Fab specific) F(ab′)2 fragment-FITC antibody recognizes Fab fragment of mouse IgG. No cross reaction is observed with the Fc fragment of mouse IgG. No reactivity is observed with human, horse or bovine serum. The antibody is purified by affinity isolation and conjugated with FITC in an alkaline reaction.

Immunogen

Purified mouse IgG; The F(ab′)2 fragment of the antibody is obtained from pepsin digested antiserum

Application

Anti-Mouse IgG (Fab specific) F(ab′)2 fragment−FITC antibody produced in goat has been used in:
• flow cytometry for fluorescence-activated cell sorting (FACS) analysis
• immunofluorescence and confocal microscopy
• immunofluorescent HSP72 detection/immunocytochemistry experiments

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Biochem/physiol Actions

Immunoglobulin G (IgG) participates in hypersensitivity type II and type III reactions. IgG2a has cytotropic properties and IgG2 molecules have the ability to activate the complement system.

Maternal IgG is transferred to fetus through the placenta that is vital for immune defence of the neonate against infections.

Other Notes

Antibody adsorbed with bovine, equine and human serum proteins.

Preparation Note

Adsorbed to reduce background staining with bovine, horse or human samples.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
nwg
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable
Protocols & Articles

Articles

Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy

How to Choose a Secondary Antibody

The following information is provided to help you decide which secondary antibody may be best for your particular application.
Keywords: Amplification, Enzyme-linked immunosorbent assay, Flow cytometry, Immobilization, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Western blot

Secondary Antibodies, Conjugates and Kits

Secondary antibodies are polyclonal or monoclonal antibodies that bind to primary antibodies or antibody fragments, such as the Fc or Fab regions. They are typically labeled with probes that make the...
Keywords: Absorption, Adsorption, Amplification, Bacterial conjugations, Cancer, Digestions, Electrophoresis, Enzyme-linked immunosorbent assay, Flow cytometry, Gel electrophoresis, Gene expression, Hormones, Immobilization, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Immunology, Immunoprecipitation, Infrared spectroscopy, Microbiology, Microscopy, Purification, Vitamins, Western blot

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Peer-Reviewed Papers
15

References

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