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  • F8527 - Monoclonal Anti-CD45−FITC/CD14-PE DUAL-TAG antibody produced in mouse

F8527 Sigma

Monoclonal Anti-CD45−FITC/CD14-PE DUAL-TAG antibody produced in mouse

clone BRA-55/UCHM-1, purified immunoglobulin, buffered aqueous solution



Related Categories Alphabetical Index, Analytical Cytology, Antibodies, Antibodies for Cell Biology, Antibodies for Immune Cell Signaling and Blood,
conjugate   FITC conjugate
  phycoerythrin (R-PE) conjugate
clone   BRA-55/UCHM-1, monoclonal
biological source   mouse
application(s)   flow cytometry: 20 μL using 1 × 106 cells
species reactivity   human
form   buffered aqueous solution
shipped in   wet ice
storage temp.   2-8°C
antibody form   purified immunoglobulin
isotype   IgG1/IgG2a
antibody product type   primary antibodies
UniProt accession no.   P08571
Gene Information   human ... CD14(929), PTPRC(5788)



Monoclonal Anti-CD45-FITC/CD14-PE DUAL-TAG antibody is suitable for flow cytometry at a concentration of 20μL using 1×106 cells.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide.


Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Legal Information

DUAL-TAG is a trademark of Sigma-Aldrich Co. LLC

Biochem/physiol Actions

CD45 is a family of single chain transmembrane glycoproteins, consisting of different isoforms, which share a common large intracellular domain. It is abundantly present in leukocyte cell surface and belongs to a novel class of enzymes, the protein tyrosine phosphatases (PTPs). It plays an essential role in thymic selection and peripheral activation of T and B cells. It is associated with various signal transduction pathways. It may also accelerate the Src family tyrosine kinases activity. an important determinant of immune tolerance.

Safety & Documentation

Safety Information

WGK Germany 
Protocols & Articles


Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy

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