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F8775 Sigma-Aldrich

Formaldehyde solution

for molecular biology, 36.5-38% in H2O

Synonym: Formalin

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Properties

Related Categories Core Bioreagents, General Reagents, Life Science Reagents for DNA/RNA Electrophoresis, Life Science Reagents for Northern and Southern Blotting, Life Science Reagents for Protein Electrophoresis,
grade   for molecular biology
vapor density   1.03 (vs air)
vapor pressure   52 mmHg ( 37 °C)
InChI Key   WSFSSNUMVMOOMR-UHFFFAOYSA-N
autoignition temp.   572 °F
contains   10-15% methanol
concentration   36.5-38% in H2O
density   1.09 g/mL at 25 °C (lit.)
suitability   suitable for (use in Formaldehyde-Agarose Gel Electrophoresis of RNA)

Description

General description

Formaldehyde (formalin) is produced by oxidation of methanol. It is made of 37% formaldehyde and impurities such as methanol, small amounts of formic acid, aldehydes and ketones. It is used as a denaturant in formaldehyde-agarose gel electrophoresis of RNA. A gel concentration of 2.2M formaldehyde is typically used. The formaldehyde concentration as supplied is approximately 13.3M.

Application

Formaldehyde solution has been used for fixing of cells and tissue sections. It has been used in the formaldehyde agarose gel electrophoresis.

Packaging

25, 500 mL in poly bottle

Analysis Note

Tested for use in formaldehyde-agarose gel electrophoresis of RNA.

Safety & Documentation

Safety Information

Signal word 
Danger
RIDADR 
UN 2209 8 / PGIII
WGK Germany 
3
RTECS 
LP8925000
Flash Point(F) 
132.8 °F
Flash Point(C) 
56 °C

Documents

Certificate of Analysis

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Certificate of Origin

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Single- & multi-channel pipettes from BRAND
Protocols & Articles

Protocols

Chromatin Immunoprecipitation Kit CHP1 Protocol

Product Description Storage/Stability Workflow Preparation Instructions Procedure Analysis Troubleshooting Guide Frequently Asked Questions Materials References Poster & Application Note
Keywords: AGE, Amplification, Cell culture, Cell disruption, Centrifugation, Chromatin immunoprecipitation, DNA purification, DNA replication, Digestions, Electrophoresis, Evaporation, Gel electrophoresis, Genomics, Homogenization, Immunoprecipitation, Individual protein Immunoprecipitation, Molecular biology, Molecular probes, Nucleic acid annealing, Nucleic acid hybridization, Polymerase chain reaction, Polymerase chain reaction - quantitative, Precipitation, Purification, Recombination, Sample preparations, Size-exclusion chromatography, Sonication, Transcription, Transduction, Whole genome amplification

Peer-Reviewed Papers
15

References

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