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F9182 Sigma-Aldrich

FSL-biotin

Synonym: FSL-CONJ(1Biotin)-SC2-L1

  • Empirical Formula (Hill Notation) C91H149N17NaO32PS

  • Molecular Weight 2079.28

  •  NACRES NA.32

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Description

Application

FSL Constructs Video Protocol

All FSL constructs disperse in biocompatible media and spontaneously and stably incorporate into cell membranes. Cells modified with FSL constructs are known as kodecytes and usually maintain their normal vitality and functionality. FSL-biotin has been specifically designed to create biotinylated live cells (kodecytes) but can also be used to modify other hydrophobic surfaces including fixed cells, virions, and solid phase surfaces. Biotinylated kodecytes can be reacted with a variety of biotin binding protein constructs including fluorescent labeled avidin and avidinylated beads/solid surfaces.

Other Notes

FSL-biotin is a KODE technology construct designed to label hydrophobic surfaces including living cells with biotin. All FSL constructs consist of three essential designable features: a functional component (F), a spacer (S), and a diacyl lipid (L). FSL-biotin is comprised of a monomer of biotin (vitamin B7) representing F, conjugated to a maleimide-bearing carboxymethylglycine based linker (SC2) in turn conjugated to an activated adipate derivative of dioleoylphosphatidylethanolamine (L1).

Legal Information

KODE Constructs are supplied by KODE Biotech Materials Limited under license from KODE Biotech Limited.

KODE is a trademark of KODE Biotech Ltd.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
3

Documents

Certificate of Analysis (COA)

Please Enter a Lot Number

Certificate of Origin (COO)

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Frequently Asked Questions

How does the storage temperature relate to shipping conditions?
The storage conditions that a Sigma-Aldrich catalog and label recommend for products are deliberately conservative. For many products, long-term storage at low temperatures will increase the time during which they are expected to remain in specification and therefore are labeled accordingly. Where short-term storage, shipping time frame, or exposure to conditions other than those recommended for long-term storage will not affect product quality, Sigma-Aldrich will ship at ambient temperature. The products sensitive to short-term exposure to conditions other than their recommended long-term storage are shipped on wet or dry ice. Ambient temperature shipping helps to control shipping costs for our customers. At any time, our customers can request wet- or dry-ice shipment, but the special handling is at customer expense if our product history indicates that the product is stable for regular shipment. See Shipping and Storage for more information.
Which document(s) contains shelf-life or expiration date information for a given product?
If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.
How do I get lot-specific information or a Certificate of Analysis?
The lot specific COA document can be found by entering the lot number above under the "Documents" section.
How do I find price and availability?
There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote. USA customers: 1-800-325-3010 or view local office numbers.
What is the Department of Transportation shipping information for this product?
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
What should I use to reconstitute Product F9182, FSL-biotin?
We recommend reconstituting 1 mg of Product F9182, FSL-biotin with 0.5 mL of saline or PBS for a concentration of 2 mg/mL.
May I use serum with the product F9182, FSL-biotin?
We do not recommend diluting the Product F9182, FSL-biotin, in lipid-containing buffers. The FSL will associate with the lipids and reduce the efficiency of insertion into cell membranes.
Will the Product F9182, FSL-biotin, label dead cells?
Product F9182, FSL-biotin, is designed to insert into the membranes of live cells. But it can also be used to label fixed cells, enveloped virions, and solid phase surfaces.
What concentration of F9182, FSL-biotin do I use to label cells?
For cell labeling we recommend using a concentration of 10-100ug/ml of F9182, FSL-biotin diluted in PBS. Label cells with FSL-biotin at a 1:1 volume to volume ratio. The exact concentration may need to be optimized for the cells and the application.
How many cells can I label with the product F9182, FSL-biotin?
The exact number of cells may vary based on cell type and cell condition. The recommended concentration to use for labeling is 10-100ug/ml (~4.8-48mM), which is mixed in a 1:1 volume with cells.
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Protocols & Articles

Protocols

FSL Construct Protocol

The following protocol describes the generic procedure for the insertion of FSL constructs (Figure 1) into biological membranes. For simplicity the protocol will only refer to cells (kodecytes), whic...
Adapted from Blake, D.A., et al. FSL Constructs: A simple method for Modifying Cell/Virion Surfaces with a Range of Biological Markers Without Affecting their Viability. J. Vis. Exp. (54), e3289, doi:10.3791/3289 (2011).
 
Keywords: Cell culture, Cell preservation, Centrifugation, Culture media, Detergents, Flow cytometry, Microscopy, Solvents

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