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F9378 Sigma-Aldrich

Ficoll® 400

Type 400-DL, lyophilized powder

Synonym: Polysucrose 400

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Description

Application

Ficoll® 400 is used together with sodium diatrizoate (Paque) to create Ficoll-Paque, a density mixture, used for the separation of cells and organelles, especially blood derived lymphocytes and monocytes. Ficoll 400 may be used for the development of new cell and organelle separation systems. Ficoll 400 is used in tissue engineering and macromolecular conformation research for the development, evaluation and use of macromolecular crowding (MMC) systems and configurations.

Used for cell separation and organelle isolation.

Packaging

5, 10, 25, 100, 500 g in poly bottle

Other Notes

A nonionic synthetic polymer of sucrose.

Dialyzed

Legal Information

Ficoll is a registered trademark of GE Healthcare

Safety & Documentation

Safety Information

Personal Protective Equipment 
RIDADR 
NONH for all modes of transport
WGK Germany 
WGK 3
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable

Documents

Certificate of Analysis (COA)

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Certificate of Origin (COO)

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Single- & multi-channel pipettes from BRAND
Protocols & Articles

Protocols

FICOLL 400*

Ficoll 400 is a highly branched polymer formed by the copolymerization of sucrose and epichlorohydrin. Ficoll 400 is completely non-ionic. Because of the abundance of hydroxyl groups, Ficoll 400 is v...
Keywords: Cell culture, Centrifugation, Culture media, Dialysis, Electrophoresis, Fractionation, Growth factors, Nucleic acid hybridization, Separation, Southern blot, Substitutions

Peer-Reviewed Papers
15

References

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