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F9429 Sigma-Aldrich

Anti-FBI-1/Pokemon (C-terminal) antibody produced in rabbit

affinity isolated antibody, buffered aqueous solution

Synonym: Anti-FBI-1, Anti-LRF, Anti-POK Erythroid Myeloid Ontogenic Factor, Anti-ZBTB7, Anti-Zinc Finger- and BTB Domain-containing Protein 7

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Properties

Related Categories Alphabetical Index, Antibodies, Antibodies against Proteins, Bioactives, Markers, and Receptors for Stem Cell Biology, Antibodies against Proteins/Bioactives/Markers/Receptors for Stem Cell Biology, Antibodies for Cell Biology,
conjugate   unconjugated
clone   polyclonal
biological source   rabbit
application(s)   immunoprecipitation (IP): 1-2 μg using A431 cell lysates
  microarray: suitable
  western blot: 0.5-1 μg/mL using K-562 cell lysates
species reactivity   human
mol wt   antigen mol wt 75 kDa (additional band at 70 kDa may be seen)
form   buffered aqueous solution
shipped in   dry ice
storage temp.   −20°C
antibody form   affinity isolated antibody
antibody product type   primary antibodies
UniProt accession no.   O95365
Gene Information   human ... ZBTB7A(51341)

Description

General description

FBI-1/Pokemon is a BTB/POZ containing zinc-finger protein. It is predominantly expressed in a subset of T-cell lymphoma and B-cell lymphomas. It is also found in breast, lung, colon, prostate, and bladder carcinomas. It was identified as a critical factor in oncogenesis and is called Pokemon (POK erythroid myeloid ontogenic factor).

Immunogen

synthetic peptide corresponding to amino acids 570-584 of human FBI-1/Pokemon, conjugated to KLH.

Application

Anti-FBI-1/Pokemon (C-terminal) antibody is suitable for immunoprecipitation at concentration of 1-2μg using A431 cell lysates, microarray and western blot at concentration of 0.5-1μg/mL using K-562 cell lysates.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, and 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Biochem/physiol Actions

FBI-1/Pokemon (C-Terminal) act as potent transcriptional repressors through the recruitment of histone deacetylases (HDACs) and subsequent chromatin remodeling. By binding to the tumor suppressor gene ARF, the Pokemon exerts its oncogenic activity.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
3

Milli-Q® Water Purification Solutions
Protocols & Articles

Articles

Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy

Protocols

Western Blot Protocol | Immunoblotting Protocol

Western Blotting refers to the electrophoretic transfer of proteins from sodium dodecyl sulfate polyacrylamide gels to sheets of PVDF or nitrocellullose membrane, followed by immunodetection of prote...
Keywords: AGE, Buffers, Cell disruption, Detergents, Dialysis, Electroblotting, Electrophoresis, Enzyme activity, Gel electrophoresis, Immunoprecipitation, PAGE, Protein extraction, Purification, Sample preparations, Western blot

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Peer-Reviewed Papers
15

References

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