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  • F9666 - Monoclonal Anti-Fibroblast Growth Factor-Acidic antibody produced in mouse

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F9666 Sigma-Aldrich

Monoclonal Anti-Fibroblast Growth Factor-Acidic antibody produced in mouse

clone FA-88, purified immunoglobulin, buffered aqueous solution

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Properties

Related Categories Alphabetical Index, Angiogenesis, Antibodies, Antibodies for Cell Biology, Antibodies for Kinase/Phosphatase Biology,
conjugate   unconjugated
clone   FA-88, monoclonal
biological source   mouse
application(s)   indirect ELISA: suitable
  neutralization: suitable
  western blot: 5 μg/mL using recombinant human FGF1 preparation
species reactivity   human, bovine
mol wt   antigen 18 kDa
form   buffered aqueous solution
shipped in   dry ice
storage temp.   −20°C
antibody form   purified immunoglobulin
isotype   IgG2a
antibody product type   primary antibodies
UniProt accession no.   P05230
Gene Information   human ... FGF1(2246)

Description

Specificity

The antibody recognizes an epitope at or near the receptor binding site of FGF1. It reacts specifically with human FGF1 of both natural and recombinant origin and does not cross-react with human FGF2.

Immunogen

recombinant human FGF1.

Physical form

Solution, 0.2 μm filtered, in phosphate buffered saline, pH 7.4.

Analysis Note

The antibody can be used for the neutralization of the biological activity of FGF1 in cell culture.

Safety & Documentation

Safety Information

Personal Protective Equipment 
RIDADR 
NONH for all modes of transport
WGK Germany 
3

Documents

Certificate of Analysis (COA)

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Milli-Q® Water Purification Solutions
Protocols & Articles

Articles

Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy

Protocols

Western Blot Protocol | Immunoblotting Protocol

Western Blotting refers to the electrophoretic transfer of proteins from sodium dodecyl sulfate polyacrylamide gels to sheets of PVDF or nitrocellullose membrane, followed by immunodetection of prote...
Keywords: AGE, Buffers, Cell disruption, Detection methods, Detergents, Dialysis, Electroblotting, Electrophoresis, Enzyme activity, Gel electrophoresis, Immunoprecipitation, PAGE, Protein extraction, Purification, Sample preparations, Western blot

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