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F9682 Sigma-Aldrich

FSL-LEa(tri)

Synonym: FSL-LEA(GALb3[Fa4]GLCNb)-SA1-L1

  • Empirical Formula (Hill Notation) C70H125N3NaO25P

  • Molecular Weight 1462.71

  •  NACRES NA.25

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Properties

Related Categories Cell Surface Modification, Glycobiology, Molecular Biology, Post-Translational Modification, Proteomics More...
Quality Level   200
assay   ≥98% (TLC)
form   solid
application(s)   thin layer chromatography (TLC): suitable
storage temp.   −20°C

Description

Application

FSL Constructs Video Protocol

All FSL constructs disperse in biocompatible media and spontaneously and stably incorporate into cell membranes. Cells modified with KODE constructs are known as kodecytes and usually maintain their normal vitality and functionality.

FSL-Lea(tri) has been specifically designed to create live cells (kodecytes) expressing blood group Lea antigens but can also be used to modify other hydrophobic surfaces including fixed cells, virions, and solid phase surfaces. This product can be used to attach reproducible and controlled levels of blood group Lea antigens to erythrocytes. These kodecytes will react with most anti-Lea blood grouping reagents including polyclonal reagents. Lea-kodecytes will also react with monoclonal anti-Leb as these reagents also cross-react with Lea.

Other Notes

FSL-Lea(tri) is a KODE technology construct designed to label hydrophobic surfaces including living cells with Lewis blood group Lea trisaccharide. All KODE FSL constructs consist of three essential designable features: a functional component (F), a spacer (S), and a diacyl lipid (L). FSL-Lea(tri) is comprised of the Lewis blood group Lea trisaccharide Galβ(Fucα4)GlcNAcβ representing F, conjugated via an O(CH2)3NH spacer (SA1) to an activated adipate derivative of dioleoyl­phos­pha­tidyl­ethanolamine (L1).

Legal Information

KODE Constructs are supplied by KODE Biotech Materials Limited under license from KODE Biotech Limited.

KODE is a trademark of KODE Biotech Ltd.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
WGK 3
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable

Documents

Certificate of Analysis (COA)

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Certificate of Origin (COO)

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Protocols & Articles

Protocols

FSL Construct Protocol

The following protocol describes the generic procedure for the insertion of FSL constructs (Figure 1) into biological membranes. For simplicity the protocol will only refer to cells (kodecytes), whic...
Adapted from Blake, D.A., et al. FSL Constructs: A simple method for Modifying Cell/Virion Surfaces with a Range of Biological Markers Without Affecting their Viability. J. Vis. Exp. (54), e3289, doi:10.3791/3289 (2011).
 
Keywords: Cell culture, Cell preservation, Centrifugation, Culture media, Detergents, Flow cytometry, Microscopy, Solvents

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