F9682 Sigma-Aldrich


Synonym: FSL-LEA(GALb3[Fa4]GLCNb)-SA1-L1

  • Empirical Formula (Hill Notation) C70H125N3NaO25P

  • Molecular Weight 1462.71

  •  NACRES NA.25



Related Categories Cell Surface Modification, Glycobiology, Molecular Biology, Post-Translational Modification, Proteomics More...
Quality Level   200
assay   ≥98% (TLC)
form   solid
application(s)   thin layer chromatography (TLC): suitable
storage temp.   −20°C



FSL Constructs Video Protocol

All FSL constructs disperse in biocompatible media and spontaneously and stably incorporate into cell membranes. Cells modified with KODE constructs are known as kodecytes and usually maintain their normal vitality and functionality.

FSL-Lea(tri) has been specifically designed to create live cells (kodecytes) expressing blood group Lea antigens but can also be used to modify other hydrophobic surfaces including fixed cells, virions, and solid phase surfaces. This product can be used to attach reproducible and controlled levels of blood group Lea antigens to erythrocytes. These kodecytes will react with most anti-Lea blood grouping reagents including polyclonal reagents. Lea-kodecytes will also react with monoclonal anti-Leb as these reagents also cross-react with Lea.

Other Notes

FSL-Lea(tri) is a KODE technology construct designed to label hydrophobic surfaces including living cells with Lewis blood group Lea trisaccharide. All KODE FSL constructs consist of three essential designable features: a functional component (F), a spacer (S), and a diacyl lipid (L). FSL-Lea(tri) is comprised of the Lewis blood group Lea trisaccharide Galβ(Fucα4)GlcNAcβ representing F, conjugated via an O(CH2)3NH spacer (SA1) to an activated adipate derivative of dioleoyl­phos­pha­tidyl­ethanolamine (L1).

Legal Information

KODE Constructs are supplied by KODE Biotech Materials Limited under license from KODE Biotech Limited.

KODE is a trademark of KODE Biotech Ltd.

Safety & Documentation

Safety Information

NONH for all modes of transport
WGK Germany 
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable


Certificate of Analysis (COA)

Please Enter a Lot Number

Certificate of Origin (COO)

Please Enter a Lot Number
Protocols & Articles


FSL Construct Protocol

The following protocol describes the generic procedure for the insertion of FSL constructs (Figure 1) into biological membranes. For simplicity the protocol will only refer to cells (kodecytes), whic...
Adapted from Blake, D.A., et al. FSL Constructs: A simple method for Modifying Cell/Virion Surfaces with a Range of Biological Markers Without Affecting their Viability. J. Vis. Exp. (54), e3289, doi:10.3791/3289 (2011).
Keywords: Cell culture, Cell preservation, Centrifugation, Culture media, Detergents, Flow cytometry, Microscopy, Solvents

Related Products

Technical Service:

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Bulk Ordering & Pricing:

Need larger quantities for your development, manufacturing or research applications?