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F9807 Sigma-Aldrich

FSL-GB3

Synonym: FSL-GB3(GALa4GALb4GLCb)-SA1-L1

  • Empirical Formula (Hill Notation) C68H122N2NaO26P

  • Molecular Weight 1437.66

  •  NACRES NA.25

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Properties

Related Categories Cell Surface Modification, Glycobiology, Molecular Biology, Post-Translational Modification, Proteomics More...
Quality Level   200
assay   ≥95% (TLC)
form   solid
storage temp.   −20°C

Description

Application

FSL Constructs Video Protocol

All FSL constructs disperse in biocompatible media and spontaneously and stably incorporate into cell membranes. Cells modified with KODE constructs are known as kodecytes and usually maintain their normal vitality and functionality.

FSL-GB3 has been specifically designed to create live cells (kodecytes) expressing GB3 antigens but can also be used to modify other hydrophobic surfaces including fixed cells, virions, and solid phase surfaces. This product has been used in vitro to inhibit HIV virions and bind to veratoxin.

Other Notes

FSL-GB3 is a KODE technology construct designed to label hydrophobic surfaces including living cells with blood group GB3 trisaccharide (also known as Pk). All KODE FSL constructs consist of three essential designable features: a functional component (F), a spacer (S), and a diacyl lipid (L). FSL-GB3 is comprised of the blood group GB3 (Pk) trisaccharide Galα4Galβ4Glcβ representing F, conjugated via an O(CH2)3NH spacer (SA1) to an activated adipate derivative of dioleoyl­phos­pha­tidyl­ethanolamine (L1).

Legal Information

KODE Constructs are supplied by KODE Biotech Materials Limited under license from KODE Biotech Limited.

KODE is a trademark of KODE Biotech Ltd.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
WGK 3
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable

Documents

Certificate of Analysis (COA)

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Certificate of Origin (COO)

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Protocols & Articles

Protocols

FSL Construct Protocol

The following protocol describes the generic procedure for the insertion of FSL constructs (Figure 1) into biological membranes. For simplicity the protocol will only refer to cells (kodecytes), whic...
Adapted from Blake, D.A., et al. FSL Constructs: A simple method for Modifying Cell/Virion Surfaces with a Range of Biological Markers Without Affecting their Viability. J. Vis. Exp. (54), e3289, doi:10.3791/3289 (2011).
 
Keywords: Cell culture, Cell preservation, Centrifugation, Culture media, Detergents, Flow cytometry, Microscopy, Solvents

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