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  • F9925 - Anti-FLIPγ/δ, C-Terminal antibody produced in rabbit

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F9925 Sigma-Aldrich

Anti-FLIPγ/δ, C-Terminal antibody produced in rabbit

1 mg/mL, affinity isolated antibody, buffered aqueous solution

Synonym: Anti-CASH, Anti-CLARP, Anti-FLAME-1, Anti-I-FLICE

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Properties

Related Categories Alphabetical Index, Antibodies, Antibodies for Apoptosis, Antibodies for Cell Biology, Antibodies to Suppressors of Apoptosis,
conjugate   unconjugated
clone   polyclonal
biological source   rabbit
application(s)   immunocytochemistry: 2 μg/mL
  immunofluorescence: 10 μg/mL
  western blot: 1:1,000
species reactivity   human, mouse
mol wt   antigen 25 kDa (FLIPδ/FLIPS)
  antigen 35 kDa (FLIPγ)
form   buffered aqueous solution
storage temp.   −20°C
antibody form   affinity isolated antibody
Quality Level   200
antibody product type   primary antibodies
concentration   1 mg/mL
UniProt accession no.   O15519
Gene Information   human ... CFLAR(8837)
mouse ... Cflar(12633)

Description

General description

In human Viral FLICE-inhibitory proteins (v-FLIPs) is identified as c-FLIP. It is composed of two death effector domains which have structural resemblance with the N-terminal half of caspase-8 and a caspase-like domain. It exist as multiple splice variants: FLIP α, β, γ and δ. Along with splice variants, it has two endogenous forms of the protein − c-FLIPlong and c-FLIPshort.

Immunogen

FLIP antibody was raised against a 19 amino acid peptide near the carboxy terminus of human FLIP.
The immunogen is located within amino acids 180 - 230 of FLIP.

Application

Anti-FLIPγ/δ, C-Terminal antibody is suitable for microarray and western blot at a dilution of 1:1,000 using HeLa, Jurkat, THP-1, A431, K562, and NIH-3T3 cell lysates.

Physical form

Solution in 0.01 M phosphate buffered saline containing 0.02% sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Biochem/physiol Actions

C-FLIP plays an important role in apoptosis signaling pathways. It acts as proapoptotic molecule or as an anti-apoptotic molecule. It has been reported that c-FLIP can interact with both FADD and caspase-8. It prevents caspase-8 recruitment and processing through DED-DED (Death Effector Domain) interaction followed by CD95-induced apoptosis.

Safety & Documentation

Safety Information

WGK Germany 
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Documents

Certificate of Analysis (COA)

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Certificate of Origin (COO)

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Protocols & Articles

Articles

Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy

Protocols

Western Blot Protocol | Immunoblotting Protocol

Western Blotting refers to the electrophoretic transfer of proteins from sodium dodecyl sulfate polyacrylamide gels to sheets of PVDF or nitrocellullose membrane, followed by immunodetection of prote...
Keywords: AGE, Buffers, Cell disruption, Detection methods, Detergents, Dialysis, Electroblotting, Electrophoresis, Enzyme activity, Gel electrophoresis, Immunoprecipitation, PAGE, Protein extraction, Purification, Sample preparations, Western blot

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Peer-Reviewed Papers
15

References

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