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FLASC Sigma-Aldrich

Adenosine 5′-triphosphate (ATP) bioluminescent somatic cell assay kit

for cellular ATP determination

  •  eCl@ss 32160414

  •  NACRES NA.26

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Description

General description

Adenosine 5′-triphosphate (ATP) bioluminescent somatic cell assay kit may be employed for the bioluminescent determination of the adenosine 5¢-triphosphate (ATP) released from a suspension of viable somatic cells. Estimates of cell concentrations may be calculated if it is assumed that the ATP content per viable cell remains constant. The number of viable somatic cells are selectively counted because, as a cell dies, its ATP is rapidly degraded.

Application

The Adenosine 5′-triphosphate (ATP) bioluminescent somatic cell assay kit has been used to accurately determine the level of ATP present in neutrophils isolated from the blood of over 100 human test patients.

Kit component only

Description

Product #

Add to Cart

FL-AAB Dilution buffer 1 mL/vial    
FL-AAM Assay mix 1 mL/vial    
FL-AAS ATP standard 1 mL/vial    
FL-SAR Somatic cell releasing reagent 1 mL/vial    
Safety & Documentation

Safety Information

Symbol 
GHS05  GHS05
Signal word 
Danger
Hazard statements 
Precautionary statements 
RIDADR 
NONH for all modes of transport
WGK Germany 
WGK 3
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable

Frequently Asked Questions

Which document(s) contains shelf-life or expiration date information for a given product?
If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.
How do I get lot-specific information or a Certificate of Analysis?
The lot specific COA document can be found by entering the lot number above under the "Documents" section.
How do I find price and availability?
There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote. USA customers: 1-800-325-3010 or view local office numbers.
What is the Department of Transportation shipping information for this product?
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
Can lysates be stored in the Somatic Cell ATP Releasing Reagent of Adenosine 5´-triphosphate (ATP) bioluminescent somatic cell assay kit, Product FLASC?
In general, no. The working Somatic Cell ATP Releasing Reagent is intended for immediate use, and not for storing ATP in.We have no specific information as to how long ATP will be stable in it.
CanAdenosine 5´-triphosphate (ATP) bioluminescent somatic cell assay kit, Product FLASC, be used for adherent cells?
We do not have a separate protocol for adherent cells. However, the somatic cell lysis reagent should be able to lyse cells that are adherent.The reagent contains enough detergent that the cells will be lysed. Once cells are lysed, it is best to assay the sample immediately.
What is the optimal dilution for tissue extracts when using Adenosine 5´-triphosphate (ATP) bioluminescent somatic cell assay kit, Product FLASC?
Sigma-Aldrich has not tested this product with tissue extracts. You will need to perform trials to determine optimal dilution of the sample.
My question is not addressed here, how can I contact Technical Service for assistance?
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Protocols & Articles
Peer-Reviewed Papers
15

References

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