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G0282 Sigma-Aldrich

Granulocyte-Macrophage Colony-Stimulating Factor from mouse

GM-CSF, from mouse, recombinant, expressed in E. coli, lyophilized powder, suitable for cell culture

Synonym: GM-CSF

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Properties

Related Categories Cell Biology, Cell Culture, Cell Signaling and Neuroscience, Cytokines and Growth Factors, Cytokines as adjuvants,
biological source   mouse
recombinant   expressed in E. coli
assay   ≥97% (SDS-PAGE)
form   lyophilized powder
potency   ≤0.2 ng/mL EC50 (corresponds to ≥5 × 106 units/mg)
quality   endotoxin tested
mol wt   ~15 kDa (125 amino acids including N-terminal methionine)
packaging   pkg of 5 and 50 μg
storage condition   avoid repeated freeze/thaw cycles
impurities   ≤1 EU/mg
color   white
solubility   water: soluble, clear, colorless
suitability   suitable for cell culture
UniProt accession no.   P01587
storage temp.   −20°C
Gene Information   mouse ... CSF2(12981), Csf2(12981)

Description

Analysis Note

The EC50 activity of mouse GM-CSF is tested in culture using murine FDP-1 cells.

Biochem/physiol Actions

Granulocyte-macrophage colony stimulating factor (GM-CSF) is a growth and differentiation factor for cells in the granulocyte, macrophage and eosinophil lineage. GM-CSF stimulates colony formation from pluripotential progenitor cells at extremely low concentrations and is an essential survival and proliferative factor for hematopoietic progenitor cells in all divisions up to maturity. It also stimulates growth in some epithelial cells and osteoclasts. GM-CSF is produced by a variety of cell types (monocytes, endothelial cells, T-cells, fibroblasts, mitogen-stimulated B-cells, and LPS-stimulated macrophages). GM-CSF is secreted as a single chain glycoprotein containing 128 amino acids for human with a conserved disulfide bond. Human and murine GM-CSF share approx. 54% sequence homology and do not cross-react in bioactivity.

Physical form

Lyophilized from 10 mM acetic acid plus 250 μg BSA.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
3

Documents

Certificate of Analysis

Certificate of Origin


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Cell Culture Guide
Protocols & Articles

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Peer-Reviewed Papers
15

References

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