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G0407 Sigma-Aldrich

Granulocyte colony-stimulating factor human

G-CSF, recombinant, expressed in E. coli, suitable for cell culture

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Properties

Related Categories Cell Biology, Cell Culture, Cell Signaling and Neuroscience, Cytokines and Growth Factors, Cytokines, Growth Factors and Chemokines for Stem Cell Differentiation,
biological source   human
recombinant   expressed in E. coli
assay   ≥95% (SDS-PAGE)
form   lyophilized powder
potency   0.01-0.1 ng/mL
quality   endotoxin tested
mol wt   18.8 kDa (175 amino acids including N-terminal methionine)
packaging   pkg of 5 and 25 μg
impurities   <1 EU/μg (LAL test)
suitability   suitable for cell culture
UniProt accession no.   P09919
storage temp.   −20°C
Gene Information   human ... CSF3(1440)

Description

Analysis Note

The biological activity is measured in a cell proliferation assay using a murine myeloblastic cell line, NFS-60.

Biochem/physiol Actions

Granulocyte-colony stimulating factor (G-CSF) is a proliferation, differentiation, survival, and activation factor for hematopoietic cells of the restricted neutrophilic granulocyte lineage. It is produced by macrophages activated by endotoxin (LPS), by monocytes activated by TNF-α with IFNγ, by fibroblasts and endothelial cells activated by IL-1 or TNF-α, and by bone marrow stromal cells activated by IL-1 or LPS. It is suggested that during the inflammatory process endotoxins stimulate tissue macrophages to produce not only G-CSF but several other cytokines, including IL-1 and TNF-α, which in turn stimulate more G-CSF release from endothelial cells and fibroblasts. G-CSF can also synergize with IL-3 to shorten the G0 period of early hematopoietic progenitors. In addition to the namesake proliferative activity, G-CSF acts on mature neutrophils to enhance their survival and to stimulate their tumorcidal activity. Human G-CSF binds and activates a 130 kD to150 kD glycoprotein single chain receptor that has been classified as a member of the hematopoietic (cytokine) receptor family, the cytokine receptor class I, or the gp130 related cytokine receptor family (although it does not apparently bind to gp130). G-CSF receptors can be found on neutrophils, myeloid leukemia cells that respond to G-CSF, bone marrow cells of neutrophilic granulocyte lineage, and on placental trophoblasts, plus a soluble form may be expressed. Two forms of human G-CSF (177 and 174 amino acids) are synthesized from a single gene by alternative splicing, but murine G-CSF is a single expressed form of 178 amino acids. Human and murine G-CSF share 73% amino acid sequence homology and full cross-reactivity.

Physical form

Lyophilized from 10 mM acetic acid containing 250 μg bovine serum albumin

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
3

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Cell Culture Guide
Protocols & Articles

Articles

Hematopoietic Cytokines

Hematopoietic cytokines are large family of extracellular ligands that stimulate hematopoietic cells to differentiate into eight principle types of blood cells. Numerous cytokines are involved in the...
Keywords: Cell proliferation, Clinical, Growth factors, Ligands

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Peer-Reviewed Papers
15

References

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