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G0762 Sigma

β-Glucuronidase from Helix pomatia

Type H-3AF, aqueous solution, ≥60,000 units/mL

Synonym: β-D-Glucuronide glucuronosohydrolase




A further processing of Type H-3 to remove agglutinin.


Many β-glucuronidases derived from mollusks also contain sulfatase activity.
Some preparations have been reported to contain acid phosphatase activity.

Unit Definition

One Sigma or modified Fishman unit will liberate 1.0 μg of phenolphthalein from phenolphthalein glucuronide per hr at 37 °C at pH 5.0 (30 min assay).

Sulfatase Unit Definition: One unit of sulfatase will hydrolyze 1.0 μmole p-nitrocatechol sulfate per hr at pH 5.0 at 37 °C.

Physical form

Aqueous solution in ~1.0 M ammonium sulfate with 3 mM sodium azide as preservative.


β-glucuronidase is used as a reporter gene in GUS assays to monitor gene expression.

Biochem/physiol Actions

Used for the hydrolysis of glucuronide conjugates in urinary metabolite analysis

β-glucuronidase catalyzes the breakdown of complex carbohydrates. In humans it converts conjugated bilirubin into the unconjugated form, making bilirubin suitable for reabsorption.

β-Glucuronidase from Helix pomatia is a mixture of enzymes derived from the Roman snail.
Optimal pH:
• Glucuronidase activity: 4.5 to 5.0
• Sulfatase activity: ~6.2
Inhibitors: D-glucuronic acid, D-galacturonic acid, D-glucaro-1, 4-lactone

Safety & Documentation

Safety Information

GHS08  GHS08
Signal word 
Hazard statements 
Precautionary statements 
NONH for all modes of transport
WGK Germany 


Certificate of Analysis

Certificate of Origin

Expore Enzymes, Proteins & Cofactors

Biomedical Applications
Protocols & Articles


Evaluation of Different Enzymes on Hydrolysis Efficiencies of Glucuronide Drug Metabolites in Urine

β-glucuronidase (GUS) enzymes are utilized to hydrolyze glucuronide (gluc) drug metabolites to the parent drug, facilitating analysis by LC-MS/MS. Here we evaluate the hydrolysis efficiency of β-gluc...
Jim Blasberg
Harkewal Singh, Kevin Ray Sigma-Aldrich, St. Louis, MO
Keywords: High performance liquid chromatography, Liquid chromatography mass spectrometry, Mass spectrometry, Metabolites


Enzymatic Assay of ß-Glucuronidase (EC from Helix Pomatia and Bovine Liver

To standardize a procedure for Enzymatic Assay of b-Glucuronidase1 at Sigma-Aldrich St. Louis.
Keywords: Enzymology

Related Content

Analytical Enzyme Beta Glucuronidase

Our β-Glucuronidases are routinely used for the enzymatic hydrolysis of glucuronides from urine1,2, plasma3,4, and other fluids5 prior to analysis by enzyme immunoassay, mass spectrometry, gas chroma...
Keywords: Bacterial conjugations, Cancer, Chromatography, Gas chromatography, High performance liquid chromatography, Immunoassay, Mass spectrometry, PAGE, Purification

Enzymes & Proteins

Application Index | Enzyme Index | Substrate Index | Inhibitor Index | Cofactor Index | Lectin Index
Keywords: Cell signaling, Diagnostic, Drug discovery, Molecular biology


在酶免疫分析、质谱分析、气相色谱、高效液相色谱或其他方法进行分析之前,我们的β-葡萄糖醛酸酶常规用于从尿液1,2、血浆3,4,和其他液体5 中酶解葡萄糖醛酸苷。 通常,每微升血浆、尿液或胆汁使用1到20单位的葡萄糖醛酸酶,用于样品中葡萄糖醛酸苷的酶解1–5。所需的确切量将取决于所使用的具体条件以及必需的经验来确定。
Keywords: Cancer

Peer-Reviewed Papers


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