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G1041 Sigma

EZBlue Gel Staining Reagent

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Description

Features and Benefits

• Premixed solution eliminates the time and effort required to prepare the stain
• Increased sensitivity ensures that low abundance proteins can be detected (as little as 5 ng)
• Rapid reaction significantly reduces the amount of time required to stain and rinse
• No solvent waste so you save time and money by eliminating hazardous material disposal

General description

Convenient, sensitive, and safe, EZBlue Coomassie Brilliant Blue G-250 colloidal protein stain improves protein electrophoresis results while significantly reducing staining time. Conveniently packaged, EZBlue requires no messy weigh-ups or additions of methanol or acid. As a colloidal stain, it reacts only with proteins, not the gel itself. Background staining is reduced, so protein bands can be visualized almost immediately. No destaining step is required, although a water wash may intensify bands and clarify the background. Most impressively, EZBlue is extremely sensitive, detecting as little as 5 ng of protein.

Other Notes

A ready-to-use Brilliant Blue G-250 based protein stain for "one step" ultrasensitive detection on polyacrylamide gels and PVDF membranes.

Application

EZBlue® Gel Staining Reagent has been used as a staining reagent in sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE).

Legal Information

EZBlue is a trademark of Sigma-Aldrich Co. LLC

Sigma-Aldrich is a registered trademark of Sigma-Aldrich Co. LLC


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Protocols & Articles

Articles

A Rapid, Streamlined Workflow for Glycan Sample Preparation and Analysis by LC-MSpngase fast asms

Comparison of PNGase F workflows for glycan release. The traditional workflow (top) involves multiple wash steps and an overnight digestion. With PNGase Fast (bottom), digestion is complete in 15 min...
Judy Boland, Amber Henry, Nicolas Caffarelli, Gordon Nicol, Jeffrey L. Turner, and Kevin Ray
Keywords: Aminations, Detergents, Digestions, Electrophoresis, High performance liquid chromatography, Mass spectrometry, PAGE, Reductions, Reductive aminations

Electrophoresis - Protein Staining Reagents

To meet the great diversity of protein analysis needs, we offer a wide selection of protein visualization (staining) reagents. EZBlue™ and ProteoSilver™, designed specifically for proteomics, also pe...
Keywords: PAGE, Proteomics

Fluorescent Detection on SDS-PAGE Gels

Identify glycoproteins with superior selectivity. Allows fluorescent detection of glycoproteins directly in PAGE gels without blotting or use of antibodies.
Glycobiology Analysis Manual, 2nd Edition
Keywords: Applications, PAGE

IP-Western: Western Blot Troubleshooting

IP-Western analysis remains a popular technique for identifying protein-protein interactions and identifying unknown proteins in a multi-protein complex. The steps include cell lysis, formation of th...
Keywords: Cell disruption, Degradations, Immunoprecipitation, Individual protein Immunoprecipitation, Nucleic acid denaturation, Precipitation, Western blot

Perparing Cell Lysates: Western Blot Troubleshooting

Often, the first step of analyzing protein expression or protein-protein interactions is to obtain a cell or tissue sample, lyse the cells, and extract proteins using extraction reagents. Total prote...
Keywords: Buffers, Cell disruption, Centrifugation, Degradations, Detergents, Gene expression, Homogenization, PAGE, Sonication, Western blot

Sample Preparation & Gel Electrophoresis: Western Blot Troubleshooting

Electric currents, wires, leads, combs, leaks… so many opportunities for trouble. But we still use gels, because electrophoresis remains an effective way to separate proteins — so that the results of...
Keywords: Buffers, Cell disruption, Centrifugation, Degradations, Electrophoresis, Gel electrophoresis, Homogenization, PAGE, Sample preparations, Sonication, Western blot

Peer-Reviewed Papers
15

References

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