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G1264 Sigma-Aldrich

Gentamicin sulfate salt

powder, BioReagent, suitable for cell culture

Synonym: Gentiomycin C

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Properties

Related Categories A - K, Aminoglycosides, Antibacterial, Antibiotics, Antibiotics A to Z,
biological source   Micromonospora purpurea
InChI Key   RDEIXVOBVLKYNT-HDZPSJEVSA-N
product line   BioReagent
form   powder
potency   ~600 μg Gentamicin per mg
color   white to off-white
solubility   H2O: 50 mg/mL (As stock solution, store at −20°C. Stable at 37°C for 5 days.)
suitability   suitable for cell culture
Mode of action   protein synthesis | interferes
antibiotic activity spectrum   Gram-negative bacteria
  Gram-positive bacteria
  mycoplasma
storage temp.   2-8°C

Description

Components

Gentamicin is an aminoglycoside complex produced by fermentation of Micromonospora purpurea or M. echinospora. It is used as the sulfate salt. There are three components, each consisting of five basic nitrogens and requiring five equivalents of sulfuric acid per mole of gentamicin base.

Biochem/physiol Actions

Mode of action: Gentamicin causes codon misreading by binding to the 30S ribosomal subunit, blocking the translocation of peptidyl-tRNA from the acceptor site to the donor site. The bactericidal effect of gentamicin on Pseudomonas aeruginosa is exerted by the binding of gentamicin to the outer membrane, where it displaces natural cations, destabilizes the membrane, and forms holes in the cell surface.

Antimicrobial spectrum: Includes Gram-negative and Gram-positive bacteria, including strains resistant to tetracycline, chloramphenicol, kanamycin and colistin, particularly strains of Pseudomonas, Proteus, Staphylococcus and Streptococcus.

Caution

Sterile solutions should be stored at 2-8°C. Solutions have also been shown to be stable at room temperature and in boiling aqueous buffers of pH 2-142.

Packaging

1, 5, 100 g in poly bottle

50, 250 mg in poly bottle

Application

Gentamicin sulfate, a broad-spectrum antibiotic, has been used as a selection agent (gentamicin-resistance gene) in cell culture and molecular biology applications. This product is recommended for use at 50 mg/L.

General description

Chemical structure: aminoglycoside

Gentamicin, also known as gentiomycin C, is an aminoglycoside, which is naturally produced by Gram-negative bacteria. This antibiotic functions by preventing protein synthesis. Gentamicin sulfate prevents the growth of both Gram positive and Gram negative bacteria, in vivo and in vitro. In tissue culture, gentamicin sulfate is used to inhibit the growth of various strains of mycoplasma.

Safety & Documentation

Safety Information

Symbol 
GHS07  GHS07
Signal word 
Warning
Hazard statements 
Precautionary statements 
Personal Protective Equipment 
RIDADR 
NONH for all modes of transport
WGK Germany 
2
RTECS 
LY2625000

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Cell Culture Guide
Protocols & Articles

Articles

Antibiotic Kill Curve

Antibiotic kill curve is a dose response experiment in which mammalian cells are subjected to increasing amounts of selection antibiotic to determine the minimum concentration of an antibiotic that c...
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Protocols

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3-D Culture is an innovative approach to modeling the morphological effects of oncogenesis and development using three-dimensional microenvironments. Extracellular matrix proteins form hydrogels unde...
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Cultrex® 3-D Culture Matrix™ Laminin I Protocol

3D Culture is a cell culture method that provides cells with the necessary structure and signaling cues to direct reconstruction of the tissue architecture, and these methods provide physiologically ...
Keywords: Cancer, Cell attachment, Cell culture, Diagnostic, Growth factors, Molecular probes, PAGE, Polymerase chain reaction

Cultrex® 3-D Culture Matrix™ Reduced Growth Factor Basement Membrane Extract, PathClear® Protocol

3D Culture is a cell culture method that provides cells with the necessary structure and signaling cues to direct reconstruction of the tissue architecture, and these methods provide physiologically ...
Keywords: Cancer, Cell culture, Diagnostic, Growth factors, Molecular probes, Polymerase chain reaction

Cultrex® Basement Membrane Extract, Type 2, PathClear® Protocol

Basement membranes are continuous sheets of specialized extracellular matrix that form an interface between endothelial, epithelial, muscle, or neuronal cells and their adjacent stroma. Basement memb...
Keywords: Adhesion, Angiogenesis, Cell attachment, Cell culture, Diagnostic, Polymerase chain reaction

Cultrex® Reduced Growth Factor Basement Membrane Extract, Type 2, PathClear® Protocol

Basement membranes are continuous sheets of specialized extracellular matrix that form an interface between endothelial, epithelial, muscle, or neuronal cells and their adjacent stroma. Basement memb...
Keywords: Adhesion, Angiogenesis, Cell attachment, Cell culture, Diagnostic, Polymerase chain reaction

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Peer-Reviewed Papers
15

References

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