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G1272 Sigma-Aldrich

Gentamicin solution

10 mg/mL in deionized water, liquid, sterile-filtered, BioReagent, suitable for cell culture

Synonym: Garamycin, Gentiomycin C

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Properties

Related Categories A - K, Aminoglycosides, Antibacterial, Antibiotic Solutions, Antibiotics,
biological source   microbial
InChI Key   RDEIXVOBVLKYNT-HDZPSJEVSA-N
sterility   sterile-filtered
product line   BioReagent
form   liquid
potency   10-12 mg per mL
concentration   10 mg/mL in deionized water
application(s)   cell culture | mammalian: suitable
impurities   endotoxin, tested
color   colorless to light yellow
suitability   suitable for cell culture
Mode of action   protein synthesis | interferes
antibiotic activity spectrum   Gram-negative bacteria
  Gram-positive bacteria
  mycoplasma
storage temp.   2-8°C

Description

Components

Gentamicin is an aminoglycoside complex produced by fermentation of Micromonospora purpurea or M. echinospora. It is used as the sulfate salt. There are three components, each consisting of five basic nitrogens and requiresing five equivalents of sulfuric acid per mole of gentamicin base.

Application

Gentamicin sulfate is a broad-spectrum antibiotic used as a selection agent (gentamicin-resistance gene) in molecular biology and cell culture applications. This product is formulated to contain 10 mg/mL Gentamicin in deionized water and is recommended for use in eukaryotic cell culture at a volume of 1 milliliter per liter.

Biochem/physiol Actions

Mode of Action: Gentamicin inhibits protein synthesis by binding to the 30S subunit of the ribosome.

Antimicrobial spectrum: Includes Gram-negative and Gram-positive bacteria, including strains resistant to tetracycline, chloramphenicol, kanamycin and colistin, particularly strains of Pseudomonas, Proteus, Staphylococcus and Streptococcus.

Caution

The product is stable at 37° for 5 days and is stored at 2-8°C.

General description

Chemical structure: aminoglycoside

Safety & Documentation

Safety Information

Symbol 
GHS07  GHS07
Signal word 
Warning
Hazard statements 
Precautionary statements 
RIDADR 
NONH for all modes of transport
WGK Germany 
3

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Cell Culture Guide
Protocols & Articles

Articles

Antibiotic Kill Curve

Antibiotic kill curve is a dose response experiment in which mammalian cells are subjected to increasing amounts of selection antibiotic to determine the minimum concentration of an antibiotic that c...
Keywords: Antibiotics, Cell culture, Evaporation, Transfection

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Peer-Reviewed Papers
15

References

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