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G1279 Sigma-Aldrich

G 418 disulfate salt

plant cell culture tested, BioReagent

Synonym: Antibiotic G418

  • CAS Number 108321-42-2

  • Empirical Formula (Hill Notation) C20H40N4O10 · 2H2SO4

  • Molecular Weight 692.71

  •  MDL number MFCD00058314

  •  PubChem Substance ID 24895058

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Properties

Related Categories A - K, Aminoglycosides, Antibacterial, Antibiotics, Antibiotics A to Z,
InChI Key   UHEPSJJJMTWUCP-NKCAIAFTSA-N
product line   BioReagent
form   powder
potency   ≥450 μg per mg (Dried basis)
application(s)   cell culture | plant: suitable
color   white to off-white
solubility   H2O: 50 mg/mL, clear to very slightly hazy, colorless to faintly yellow
suitability   plant cell culture tested
Featured Industry   Agriculture
Mode of action   protein synthesis | interferes
antibiotic activity spectrum   Gram-negative bacteria
storage temp.   2-8°C

Description

Application

G418 disulfate salt is an aminoglycoside antibiotic similar in structure to gentamicin used for selection and maintenance of prokaryotic and eukaryotic cells transfected with a iNOS promoter construct and neomycin resistance gene†. Optimal concentrations for selection and maintenance vary by cell lines. Bacteria and algae require 5 μg/mL or less while animal cells may require 300-500 μg/mL.

Caution

The product is stable for three years when stored at 2-8°C.

Packaging

1, 5 g in glass bottle

250 mg in glass bottle

Preparation Note

G418 powder is soluble in water at 50 mg/mL.

General description

Chemical structure: aminoglycoside

Safety & Documentation

Safety Information

Symbol 
GHS07  GHS07
Signal word 
Warning
Hazard statements 
Precautionary statements 
RIDADR 
NONH for all modes of transport
WGK Germany 
3

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Protocols & Articles

Articles

Antibiotic Kill Curve

Antibiotic kill curve is a dose response experiment in which mammalian cells are subjected to increasing amounts of selection antibiotic to determine the minimum concentration of an antibiotic that c...
Keywords: Antibiotics, Cell culture, Evaporation, Transfection

Protocols

Antibiotics - Plant Tissue Culture Protocol

In general, antibiotics require storage in a refrigerator or freezer. Aminoglycosides (e.g. kanamycin) are hygroscopic and should be stored in a desiccator. Storage of many powdered antibiotics at -2...
Keywords: Adsorption, Antibiotics, Antifungals, Condensations, Molecular biology

Related Content

Antibiotics Selection Guide

The Antibiotic Selection Guide enables researchers to spend more time on their research discoveries and less time seeking established antibiotic information. Link to hundreds of antibiotics for conta...
Keywords: Antibiotics, Cell biology, Cell culture, Genetic, High performance liquid chromatography, Reversed-phase chromatography

Plant Tissue Culture Products | Agriculture

Our combination of quality products, services and global business capabilities help you to maintain reagent consistency while ensuring suitability. Sigma-Aldrich allows you to standardize reagents ac...
Keywords: Antibiotics, Cell culture, Culture media, Gas chromatography, High performance liquid chromatography, Mass spectrometry, Vitamins, transformation

Peer-Reviewed Papers
15

References

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