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G1393 Sigma-Aldrich

Gelatin solution

Type B, 2% in H2O, tissue culture grade, BioReagent, suitable for cell culture

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Properties

Related Categories Attachment Factors, Attachments Factors, Cell Culture, Core Bioreagents, Life Science Reagents for Cell Culture,
biological source   bovine hide
sterility   sterile
type   Type B
product line   BioReagent
form   solution
packaging   pkg of 100 mL
  pkg of 20 mL
concentration   2% in H2O, tissue culture grade
surface coverage   100‑200 μg/cm2
impurities   endotoxin, tested
suitability   suitable for cell culture
storage temp.   2-8°C

Description

General description

Gelatin is a hydrocolloid and is rich in glycine, proline and hydroxyproline, which imparts structural stability. It is synthesized from the alkaline digestion of collagen from bovine skin and is referred as type B. Gelatin has wide applications in food industry. It takes up random coil structure after digestion from the triple helical collagen. The most common source for industrial production of gelatin is slaughter byproducts. The type A gelatin and type B differ in their isoelectric pH.

Application

Gelatin solution has been used for coating of cell culture plates or dishes used for embryonic stem cell culture, testicular cell culture and neural rosettes.

Biochem/physiol Actions

Gelatin has gelling property and displays surface behaviour for use in foams and adhesions. It also serves as a clarification agent in fruit based juices. It is a digestible protein and serves as a binding agent, thickener, stabilizer and emulsifier in food industry.

Components

Gelatin is a heterogeneous mixture of water-soluble proteins of high average molecular masses, present in collagen. Proteins are extracted by boiling the relevant skin, tendons, ligaments, bones, etc. in water. Type A gelatin is derived from acid-cured tissue. Type B is derived from lime-cured tissue.

Caution

Dry gelatin, when stored in airtight containers at room temperature, will remain unchanged for many years. When heated at 100°C in the presence of air, it swells becomes soft and disintegrates to a carbonaceous mass with evolution of pyridine bases and ammonia.

Preparation Note

This product is derived lime-cured tissue bovine skin, and is a 2% solution in water.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
2

Documents

Certificate of Analysis

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Certificate of Origin

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Cell Culture Guide
Protocols & Articles

Articles

3d Cell Culture: Tools and Techniques

Cell culture techniques are ubiquitous in areas of developmental biology, drug discovery, regenerative medicine and protein production. Since the introduction of cell culture techniques, cells have b...
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Attachment Factors for 3-Dimensional Cell Culture

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BioFiles 2008, 3.8, 16.
Keywords: Adhesion, Angiogenesis, Biomaterials, Cancer, Cell biology, Cell culture, Clinical, Gene expression, Genetic, Genetics, Genomics, Growth factors, Hormones, Molecular biology, Morphogenesis

Cancer Stem Cells: New Targets for Cancer Therapy

Interested in receiving more content like this? Join over 8,000 of your peers who have already signed up for the Cell Informer newsletter.
Keywords: Cancer, Cell attachment, Cell culture, Cell proliferation, Gastrointestinal, Gene expression, Transcription, transformation

Gelatin Cell Attachment Protocol

From our library of Articles, Sigma-Aldrich presents Gelatin Cell Attachment Protocol
George Sitterley
BioFiles 2008, 3.8, 10.
Keywords: Sterilizations

Gelatin

Gelatin is a heterogeneous mixture of water-soluble proteins of high average molecular weights, present in collagen. The proteins are extracted by boiling skin, tendons, ligaments, bones, etc. in wat...
George Sitterley
BioFiles 2008, 3.8, 10.
Keywords: Biomaterials, Cell attachment, Cell culture, Culture media, Enzyme-linked immunosorbent assay, Hemostatics, Immunohistochemistry, Pharmaceutical, Polymerase chain reaction, Veterinary, Western blot

What is the Extracellular Matrix?

The extracellular matrix (ECM) is secreted by cells and surrounds them in tissues. It has long been understood to be the structural support for cells since its characteristics set the characteristics...
Keywords: Adhesion, Cell culture, Cellular processes, Epimerizations, Growth factors, Immobilization, Sulfations

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Peer-Reviewed Papers
15

References

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