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G1397 Sigma-Aldrich

Gentamicin solution

50 mg/mL in deionized water, liquid, sterile-filtered, BioReagent, suitable for cell culture

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Properties

Related Categories A - K, Aminoglycosides, Antibacterial, Antibiotic Solutions, Antibiotics,
biological source   microbial
InChI Key   RDEIXVOBVLKYNT-HDZPSJEVSA-N
sterility   sterile-filtered
product line   BioReagent
form   liquid
potency   50-60 mg per mL
concentration   50 mg/mL in deionized water
application(s)   cell culture | mammalian: suitable
impurities   endotoxin, tested
color   colorless to light yellow
density   1.000 g/cm3
suitability   suitable for cell culture
Mode of action   protein synthesis | interferes
antibiotic activity spectrum   Gram-negative bacteria
  Gram-positive bacteria
  mycoplasma
storage temp.   2-8°C

Description

General description

Chemical structure: aminoglycoside

Gentamicin, also known as gentiomycin C, is an aminoglycoside, which is naturally produced by Gram-negative bacteria. This antibiotic functions by preventing protein synthesis. Gentamicin sulfate prevents the growth of both Gram positive and Gram negative bacteria, in vivo and in vitro. In tissue culture, gentamicin sulfate is used to inhibit the growth of various strains of mycoplasma.

Application

Gentamicin sulfate is a broad-spectrum antibiotic used as a selection agent (gentamicin-resistance gene) in molecular biology and cell culture applications. This product is formulated to contain 50 mg/mL Gentamicin in deionized water and is recommended for use in eukaryotic cell culture at a volume of 1 milliliter per liter.
Gentamicin sulfate solution has been used as a component of different types of culture media.

Biochem/physiol Actions

Mode of Action: Gentamicin inhibits protein synthesis by binding to the 30S subunit of the ribosome.

Antimicrobial spectrum: Includes Gram-negative and Gram-positive bacteria, including strains resistant to tetracycline, chloramphenicol, kanamycin and colistin, particularly strains of Pseudomonas, Proteus, Staphylococcus and Streptococcus.

Components

Gentamicin is an aminoglycoside complex produced by fermentation of Micromonospora purpurea or M. echinospora. It is used as the sulfate salt. There are three components, each consisting of five basic nitrogens and requiresing five equivalents of sulfuric acid per mole of gentamicin base.

Caution

The product is stable at 37° for 5 days and is stored at 2-8°C.

Safety & Documentation

Safety Information

Symbol 
GHS07  GHS07
Signal word 
Warning
Hazard statements 
Precautionary statements 
RIDADR 
NONH for all modes of transport
WGK Germany 
3

Documents

Certificate of Analysis

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Certificate of Origin

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Cell Culture Guide
Protocols & Articles

Articles

Antibiotic Kill Curve

Antibiotic kill curve is a dose response experiment in which mammalian cells are subjected to increasing amounts of selection antibiotic to determine the minimum concentration of an antibiotic that c...
Keywords: Antibiotics, Cell culture, Evaporation, Transfection

Human Renal Proximal Tubule Epithelial Cells (RPTEC) as an Essential Advanced Model to Express Kidney Drug Transporters & Toxicity

Human renal proximal tubule epithelial cells (RPTECs) are commonly used to predict human renal drug permeability and to investigate drug efflux. However, current methods for investigating drug-transp...
Keywords: Cell culture, Chromatin immunoprecipitation, Gene expression, High performance liquid chromatography, Liquid chromatography mass spectrometry, Mass spectrometry, Polymerase chain reaction

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Peer-Reviewed Papers
15

References

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