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G1881 Sigma-Aldrich

α-Glycerophosphate Dehydrogenase-Triosephosphate Isomerase from rabbit muscle

Type III, ammonium sulfate suspension, TPI 750-2000 units/mg protein, GDH 75-200 units/mg protein (biuret)

Synonym: α-GDH-TPI

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Description

Application

α-Glycerophosphate Dehydrogenase-Triosephosphate Isomerase from rabbit muscle has been used in spectrophotometric aldolase assay and fructose 2,6 bisphosphate assay.

α-glycerophosphate dehydrogenase was used in 2-deoxy-ribose 5-phosphate aldolase (DERA) cleavage (retroaldol) assay.

Biochem/physiol Actions

α-Glycerophosphate dehydrogenase (α-GPDH) has the ability to produce reactive oxygen species (ROS), that is induced by Ca2+.

α-glycerophosphate dehydrogenase catalyzes the conversion of dihydroxyacetone to glycerol phosphate.

Packaging

(Sold on the basis of α-GDH units.)

Unit Definition

(α-GDH) One unit will convert 1.0 μmole of dihydroxyacetone phosphate to α-glycerophosphate per min at pH 7.4 at 25 °C.

(TPI) One unit will convert 1.0 μmole of D-glyceraldehyde 3-phosphate to dihydroxyacetone phosphate per min at pH 7.6 at 25 °C.

Physical form

Mixed crystalline suspension in 3.2 M (NH4)2SO4, with 0.05 g/L EDTA, pH 6.0

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
WGK 2
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable

Documents

Certificate of Analysis (COA)

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Certificate of Origin (COO)

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Protocols & Articles

Articles

Ammonium Sulfate Suspensions - Technical Note

Note that for an ammonium sulfate suspension, most of the enzyme will be in solid form. It is likely that only negligible amounts of enzyme will be in the ammonium sulfate solution.
Keywords: Enzyme activity

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Peer-Reviewed Papers
15

References

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