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G2267 Sigma-Aldrich

Glyceraldehyde-3-phosphate Dehydrogenase from rabbit muscle

lyophilized powder, ≥75 units/mg protein

Synonym: D-Glyceraldehyde 3-phosphate:NAD+ oxidoreductase (phosphorylating), GAPDH

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Properties

Related Categories 1.2.x.x Acting on aldehydes or oxo groups, 1.x.x.x Oxidoreductases, Biochemicals and Reagents, Cell Biology, Enzyme Class Index,
form   lyophilized powder
composition   Protein, ≥80% biuret
foreign activity   3-Phosphoglyceric phosphokinase ≤0.05%
  lactic dehydrogenase, myokinase, and pyruvate kinase ≤0.01%
  triosephosphate isomerase ≤0.1%
shipped in   wet ice
storage temp.   −20°C

Description

General description

Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) is a multifunctional protein. It is basically called as a glycolytic enzyme.

Application

Glyceraldehyde-3-phosphate Dehydrogenase from rabbit muscle has been used:
• for measurements of glyceraldehyde-3-phosphate dehydrogenase (GAPDH) activity assay
• to generate a linear standard curve to analyse GAPDH activity in the experimental samples collected from mice
• in anti-aggregation assays
• in purified GAPDH studies

Packaging

1000, 5000, 10000 units in poly bottle

500 units in glass bottle

Biochem/physiol Actions

Glyceraldehyde-3-phosphate dehydrogenase catalyzes the conversion of glyceraldehyde-3-phosphate to 1,3-bisphosphoglycerate as part of glycolysis. It has also been shown to have roles in initiation of apoptosis, transcription activation and the shuttling of ER to Golgi vesicles<<<18,New>>>.

Glyceraldehyde-3-phosphate dehydrogenase catalyzes the conversion of glyceraldehyde-3-phosphate into D-glycerate-1,3-bisphosphate as part of the glycolysis pathway.

Unit Definition

One unit will reduce 1.0 μmole of 3-phosphoglycerate to D-glyceraldehyde 3-phosphate per min in a coupled system with 3-phosphoglyceric phosphokinase at pH 7.6 at 25 °C.

Physical form

Lyophilized powder containing citrate buffer salts

Safety & Documentation

Safety Information

Personal Protective Equipment 
RIDADR 
NONH for all modes of transport
WGK Germany 
WGK 3
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable

Documents

Certificate of Analysis (COA)

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Certificate of Origin (COO)

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Protocols & Articles

Articles

Insulin Signaling and Energy Homeostasis

Glucose metabolism is regulated by the opposing actions of insulin and glucagon. Insulin is released from pancreatic ß cells in response to high blood glucose levels and regulates glucose metabolism ...
Linda Stephenson, Ph.D.
Biofiles v6 n4, 2011
Keywords: Apoptosis, Biological processes, Carboxylations, Catalog, Diabetes, Gene expression, Gluconeogenesis, Glycolysis, Hormones, Metabolism, Metabolites, Transcription, Transduction, Type

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Peer-Reviewed Papers
15

References

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