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G2626 Sigma-Aldrich

L-Glutamic Dehydrogenase from bovine liver

Type II, ammonium sulfate suspension, ≥35 units/mg protein

Synonym: L-GLDH, L-Glutamate:NAD[P]+ Oxidoreductase (deaminating), Glutamate Dehydrogenase from bovine liver

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Properties

Related Categories 1.4.x.x Acting on CH-NH2, 1.x.x.x Oxidoreductases, Biochemicals and Reagents, Cell Biology, Enzyme Class Index,
Quality Level   200
type   Type II
form   ammonium sulfate suspension
mol wt   310-350 kDa
shipped in   wet ice
storage temp.   2-8°C
Gene Information   cow ... GLUD1(281785)

Description

Application

L-glutamic dehydrogenase was used to catalyze the conversion of isocitrate into α-ketoglutarate and carbon dioxide.

Biochem/physiol Actions

Mammalian forms of this enzyme, including this bovine form, can use either NADP(H) or NAD(H) as coenzymes. L-glutamic dehydrogenase plays a unique role in mammalian metabolism. The reverse reaction catalyzed by this enzyme is the only pathway by which ammonia can become bound to the α-carbon atom of an α-carboxylic acid and thus, is the only source of de novo amino acid synthesis in mammalian species.

The bovine enzyme is characterized by three sets of properties:
• It has a reversible concentration-dependent association, producing higher molecular weight forms.
• Forms tight enzyme-reduced coenzyme-substrate ternary complexes whose rates of dissociation modulate the steady-state reaction rates.
• Exhibits a wide variety of effects from the binding of any of a number of nucleotide modifiers.

L-glutamic dehydrogenase catalyzes the conversion of glutamate to α-ketoglutarate.

Unit Definition

One unit will reduce 1.0 μmole of α-ketoglutarate to L-glutamate per min at pH 7.3 at 25 °C, in the presence of ammonium ions.

Physical form

Suspension in 50% saturated ammonium sulphate solution

Analysis Note

Protein determined by biuret.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
1

Documents

Certificate of Analysis (COA)

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Certificate of Origin (COO)

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Protocols & Articles

Articles

HPLC Analysis of Protein Standards (1) on BIOshell™ A400 Protein C4

From our library of Articles, Sigma-Aldrich presents HPLC Analysis of Protein Standards (1) on BIOshell™ A400 Protein C4
Keywords: Chromatography, High performance liquid chromatography, Reversed-phase chromatography

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Peer-Reviewed Papers
15

References

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