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G2751 Sigma

Glutamic-Oxalacetic Transaminase from porcine heart

Type I, ammonium sulfate suspension, 200-500 units/mg protein

Synonym: L-Aspartate:2-oxoglutarate aminotransferase, Aspartate Aminotransferase, GOT



Related Categories 2.6.x.x Nitrogenous groups, 2.x.x.x Transferases, Application Index, Biochemicals and Reagents, Cell Biology,
type   Type I
form   ammonium sulfate suspension
foreign activity   glutamic-pyruvic transaminase ≤0.03%
  lactic dehydrogenase ≤0.01%
  malic dehydrogenase ≤0.01%
shipped in   wet ice
storage temp.   2-8°C


Analysis Note

Protein determined by biuret.


10000 units in serum bottle

Unit Definition

One unit will convert 1.0 μmole of α-ketoglutarate to L-glutamate per min at pH 7.5 at 37 °C, in the presence of L-aspartic acid. One unit is equivalent to ~2,000O.D. (Karmen) units at 25 °C.

Physical form

Suspension in 3.0 M (NH4)2SO4 containing 0.05 M maleate and 2.5 mM α-ketoglutarate, pH 6.0

Price and Availability

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Safety Information

NONH for all modes of transport
WGK Germany 
Protocols & Articles


Ammonium Sulfate Suspensions - Technical Note

Note that for an ammonium sulfate suspension, most of the enzyme will be in solid form. It is likely that only negligible amounts of enzyme will be in the ammonium sulfate solution.
Keywords: Enzyme activity

Plasma Derived Proteins and Enzymes

Despite their complexity, blood and plasma are abundant biological resources for the discovery of drug targets and biomarkers for human disease. It is estimated that plasma may contain as many as 40,...
BioFiles 2006, 1.5, 2.
Keywords: Applications, Gas chromatography, Type

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Application Index | Enzyme Index | Substrate Index | Inhibitor Index | Cofactor Index | Lectin Index
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