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  • G2791 - Monoclonal Anti-Grb-2 antibody produced in mouse

G2791 Sigma

Monoclonal Anti-Grb-2 antibody produced in mouse

~2 mg/mL, clone GRB-232, purified immunoglobulin, buffered aqueous solution



Related Categories Alphabetical Index, Antibodies, Antibodies for Cell Biology, Antibodies for Kinase/Phosphatase Biology, GR-GZ,
species reactivity   human, mouse, rat
application(s)   immunocytochemistry: suitable
  immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
  immunoprecipitation (IP): suitable
  indirect ELISA: suitable
  microarray: suitable
  western blot: 1-2 μg/mL using a whole extract of cultured human acute T-cell leukemia Jurkat cells
clone   GRB-232, monoclonal
concentration   ~2 mg/mL
antibody form   purified immunoglobulin
form   buffered aqueous solution
isotype   IgG3
mol wt   antigen mol wt 24 kDa
shipped in   dry ice
storage temp.   −20°C
Gene Information   human ... GRB2(2885)
mouse ... Grb2(14784)
rat ... Grb2(81504)
biological source   mouse
conjugate   unconjugated



synthetic peptide (a.a. 200-217) corresponding to the C-terminal region of human, rat and mouse Grb2.

General description

Grb2 is an adapter protein with one Src homology 2 (SH2) domain and two SH3 domains that facilitate protein-protein interactions. grb2 is highly conserved across species and is critical for development events such as epithelial morphogenesis, cell motility and vasculogenesis. Grb2 is closely associated with Sos and activates Ras and the MAPK cascade. Grb2 interacts with signalling proteins involved in T-cell development, B-cell activation and development and autoimmunity. Recent reports implicates Grb2 role in several oncogenic signalling pathways that lead to CML and breast cancer.
Monoclonal Anti-Grb2 reacts specifically with Grb2 (24 kDa).

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Preparation Note

Purified from culture supernatant of hybridoma cells grown in a bioreactor.


Anti-Grb-2 antibody may be used at a working concentration of 1-2 μg/mL for detection by immunoblotting in whole cell extracts of human T cell leukemia, Jurkat cells. Detection is also possible in extracts of murine spermatozoa by immunoblotting at a working dilution of 1:1000. The antibody is suitable for indirect ELISA, immunocytochemistry, immunohistochemistry, immunoprecipitation and protein microarray.

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Western Blot Protocol | Immunoblotting Protocol

Western Blotting refers to the electrophoretic transfer of proteins from sodium dodecyl sulfate polyacrylamide gels to sheets of PVDF or nitrocellullose membrane, followed by immunodetection of prote...
Keywords: AGE, Buffers, Cell disruption, Detergents, Dialysis, Electroblotting, Electrophoresis, Enzyme activity, Gel electrophoresis, Immunoprecipitation, PAGE, Protein extraction, Purification, Sample preparations, Western blot

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