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G4252 Sigma-Aldrich

Glyoxalase I from Saccharomyces cerevisiae

Grade IV, buffered aqueous glycerol solution, ≥400 units/mg protein

Synonym: S-Lactoyl-glutathione methylglyoxal-lyase (isomerizing)

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Properties

Related Categories 4.4.x.x C-S bonds, 4.x.x.x Lyases, Biochemicals and Reagents, Cell Biology, Enzyme Class Index,
Quality Level   200
type   Grade IV
form   buffered aqueous glycerol solution
foreign activity   glyoxalase II ≤1%
storage temp.   2-8°C

Description

General description

Glyoxalase detoxification system consists of glyoxalase (GLO)-I and GLO-II. GLO-I is a cytosolic, 42 kDa, dimeric Zn2+ metalloenzyme.

Packaging

500, 1000, 2500 units in glass bottle

Biochem/physiol Actions

Glyoxalase I is universally expressed and involved in the protection against cellular damage due to cytotoxic metabolites such as advanced glycation end products (AGEs). It is an integral component of the detoxification system, catalyzing the conversion of reactive, acyclic a-oxoaldehydes into the corresponding a-hydroxyacids in a glutathione-dependent manner.

Unit Definition

One unit will form 1.0 μmole of S-lactoylglutathione from methylglyoxal and reduced glutathione per min at pH 6.6 at 25 °C.

Physical form

Solution in 50% glycerol, 0.4 M (NH4)2SO4 and 0.002 M KH2PO4 pH 6.5

Analysis Note

Protein determined by biuret.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
WGK 3
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable

Documents

Certificate of Analysis (COA)

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Certificate of Origin (COO)

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Protocols & Articles

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Peer-Reviewed Papers
15

References

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